caa a22 4500 469115009 CHVBK 20180323133122.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00731094 doi (NATIONALLICENCE)springer-10.1007/BF00731094 Serum immunoassay of a small cell lung cancer associated ganglioside: development of a sensitive scintillation proximity assay [Elektronische Daten] [Tina Pallesen, Annette Vangsted, Lars Drivsholm, Henrik Clausen, Jesper Zeuthen, Håkan Wallin] We here report an enzyme linked immunosorbent assay (ELISA) and a scintillation proximity assay (SPA) for detection of the ganglioside FucGM1 in sera from small cell lung cancer (SCLC) patients. The SPA was more sensitive and reproducible than the ELISA. In this assay, monoclonal antibodies specific for FucGM1 were bound to SPA particles and incubated with labelled FucGM1 and 100 µl test-serum overnight, and counted in a β-counter. The sensitivity was 0.2 ng. Seven out of twenty sera from SCLC patients were positive, whereas none of twenty sera from healthy individuals were positive for FucGM1. The SPA was more sensitive than the previously reported HPTLC as well as a direct ELISA. Chapman & Hall, 1992 SCLC nationallicence FucGM1 nationallicence sera nationallicence immunoassay nationallicence ELISA nationallicence scintillation proximity assay nationallicence MAb : monoclonal antibody nationallicence SPA : scintillation proximity assay nationallicence HPTLC : high performance thin layer chromatography nationallicence SCLC : small cell lung cancer nationallicence FucGM1 : Fucα1-2Galβ1-3GalNAcβ1-4(NeuAcα2-3)-Galβ1-4Glcβ1-1Cer nationallicence ELISA : enzyme linked immunosorbent assay nationallicence FCS : foetal calf serum nationallicence PBS : phosphate buffered saline nationallicence Pallesen Tina Department of Oncology, State University Hospital, Copenhagen, Denmark aut Vangsted Annette Department of Oncology, State University Hospital, Copenhagen, Denmark aut Drivsholm Lars Department of Oncology, State University Hospital, Copenhagen, Denmark aut Clausen Henrik School of Dentistry, University of Copenhagen, Copenhagen, Denmark aut Zeuthen Jesper Department of Oncology, State University Hospital, Copenhagen, Denmark aut Wallin Håkan Department of Environmental Carcinogenesis, Danish Cancer Society, Copenhagen, Denmark aut Glycoconjugate Journal Kluwer Academic Publishers 9/6(1992-12-01), 331-335 0282-0080 9:6<331 1992 9 10719 https://doi.org/10.1007/BF00731094 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00731094 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pallesen Tina Department of Oncology, State University Hospital, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Vangsted Annette Department of Oncology, State University Hospital, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Drivsholm Lars Department of Oncology, State University Hospital, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Clausen Henrik School of Dentistry, University of Copenhagen, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Zeuthen Jesper Department of Oncology, State University Hospital, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Wallin Håkan Department of Environmental Carcinogenesis, Danish Cancer Society, Copenhagen, Denmark aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/6(1992-12-01), 331-335 0282-0080 9:6<331 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer