caa a22 4500 469115157 CHVBK 20180323133122.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00780759 doi (NATIONALLICENCE)springer-10.1007/BF00780759 Determination of sequence and linkage of tissue oligosaccharides in caprine β-mannosidosis by fast atom bombardment, collisionally activated dissociation tandem mass spectrometry [Elektronische Daten] [Douglas Gage, Eileen Rathke, Catherine Costello, Margaret Jones] Fast atom bombardment, collisionally activated dissociation tandem mass spectrometry (FAB-CAD-MS/MS), combined withp-aminobenzoic acid ethyl ester (ABEE) derivatization, were used to confirm the sequence and linkage pattern of subnanomolar amounts of the previously characterized three major thyroid gland oligosaccharides accumulated in caprine β-mannosidosis. Positive ion FAB-CAD-MS/MS of both the [M + H]+ and [M + Na]+ ions from the ABEE derivatized oligosaccharides produced product ions derived from cleavage of the glycosidic bonds which allowed the sequences to be determined. Several fragments resulting from cleavages across the sugar ring permitted the assignment, in some cases, of the linkage positions between the sugar residues. The natriated molecule yielded several fragments of this type which were not observed when the protonated molecule was selected as the precursor ion. Use of these techniques gave the complete sequence and linkage characterization of the disaccharide and complete sequence and partial linkage information for the two higher oligosaccharides. Chapman & Hall, 1992 β-Mannosidosis oligosaccharides nationallicence sequence and linkage determination nationallicence ABEE derivatives nationallicence Gage Douglas MSU-NIH Mass Spectrometry Facility, Department of Biochemistry, Michigan State University, 48824, East Lansing, MI, USA aut Rathke Eileen Department of Pathology, Michigan State University, 48824, East Lansing, MI, USA aut Costello Catherine NIH Mass Spectrometry Facility, Department of Chemistry, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut Jones Margaret Department of Pathology, Michigan State University, 48824, East Lansing, MI, USA aut Glycoconjugate Journal Kluwer Academic Publishers 9/3(1992-06-01), 126-131 0282-0080 9:3<126 1992 9 10719 https://doi.org/10.1007/BF00780759 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00780759 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gage Douglas MSU-NIH Mass Spectrometry Facility, Department of Biochemistry, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Rathke Eileen Department of Pathology, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Costello Catherine NIH Mass Spectrometry Facility, Department of Chemistry, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut NATIONALLICENCE 700 1- Jones Margaret Department of Pathology, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/3(1992-06-01), 126-131 0282-0080 9:3<126 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer