caa a22 4500 469115173 CHVBK 20180323133122.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00780760 doi (NATIONALLICENCE)springer-10.1007/BF00780760 N -Glycosylation of membrane glycoproteins in retinol-deficient rat liver [Elektronische Daten] [R. Tauber, R. Nuck, W. Gerok, R. Büchsel, E. Köttgen, W. Lohle, Ch. Karasiewicz, W. Reutter] The effect of vitamin A deficiency onN-linked oligosaccharides of membrane glycoproteins was studied in rat liver in order to evaluate the suggested role of retinol in proteinN-glycosylation. First, oligosaccharides of newly synthesized glycoproteins from rough endoplasmic reticulum of vitamin A deficient liver were compared with that of pair-fed controls. Oligosaccharides were metabolically labelled withd-[2-3H]mannose, released from the glycoproteins with endoglycosidase H, purified by reversed phase HPLC and ion exchange chromatography, and were reduced with sodium borohydride. HPLC fractionation of the oligosaccharide alditols showed that the glycoproteins carried mainly four oligosaccharide species, Glc1Man9GlcNAc2, Man9GlcNAc2, Man8GlcNAc2 and Man7GlcNAc2, in identical relative amounts in the vitamin A deficient and the control tissue. In particular, no increase in the proportion of short chain oligosaccharides was noted in vitamin A deficient liver. Second, the number ofN-linked oligosaccharides was estimated in dipeptidylpeptidase IV (DPP IV), a major glycoprotein constituent of the hepatic plasma membrane, comparing the newly synthesized glycoprotein from rough endoplasmic reticulum and the mature form of DPP IV from the plasma membrane. No evidence was obtained that retinol deficiency caused incomplete glycosylation of this membrane glycoprotein. From these data, the suggested role of retinol as a cofactor involved in the synthesis ofN-linked oligosaccharides of glycoproteins must be questioned. Chapman & Hall, 1992 Vitamin A nationallicence retinol nationallicence N -linked oligosaccharides nationallicence membrane glycoproteins nationallicence dipeptidylpeptidase IV nationallicence DolP : Dolichyl phosphate nationallicence DolPP : dolichyl pyrophosphoryl nationallicence RetPMan : retinyl phosphate mannose nationallicence DPP IV : dipeptidyl peptidase IV (EC 3.4.14.5) nationallicence endo H : endo-β-N-acetylglucosaminidase H (EC 3.2.1.96) nationallicence endo F : endo-β-N-acetylglucosaminidase F (EC 3.2.1.96) nationallicence SDS-PAGE : sodium dodecyl sulfate polyacrylamide gel electrophoresis nationallicence Tauber R. Institut für Klinische Chemie und Biochemie, Freie Universität Berlin, Spandauer Damm 130, D-1000, Berlin 19, Germany aut Nuck R. Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33, Germany aut Gerok W. Medizinische Universitätsklinik Freiburg, Hugstetter Straße 55, D-7800, Freiburg, Germany aut Büchsel R. Medizinische Klinik III der Rheinisch-Westfälischen Technischen Hochschule, Pauwelsstraße, D-5100, Aachen, Germany aut Köttgen E. Institut für Klinische Chemie und Biochemie, Freie Universität Berlin, Spandauer Damm 130, D-1000, Berlin 19, Germany aut Lohle W. Universitäts-HNO-Klinik der Universität Freiburg, Kilianstraße, D-7800, Freiburg, Germany aut Karasiewicz Ch Institut für Klinische Chemie und Biochemie, Freie Universität Berlin, Spandauer Damm 130, D-1000, Berlin 19, Germany aut Reutter W. Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33, Germany aut Glycoconjugate Journal Kluwer Academic Publishers 9/3(1992-06-01), 132-140 0282-0080 9:3<132 1992 9 10719 https://doi.org/10.1007/BF00780760 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00780760 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tauber R. Institut für Klinische Chemie und Biochemie, Freie Universität Berlin, Spandauer Damm 130, D-1000, Berlin 19, Germany aut NATIONALLICENCE 700 1- Nuck R. Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33, Germany aut NATIONALLICENCE 700 1- Gerok W. Medizinische Universitätsklinik Freiburg, Hugstetter Straße 55, D-7800, Freiburg, Germany aut NATIONALLICENCE 700 1- Büchsel R. Medizinische Klinik III der Rheinisch-Westfälischen Technischen Hochschule, Pauwelsstraße, D-5100, Aachen, Germany aut NATIONALLICENCE 700 1- Köttgen E. Institut für Klinische Chemie und Biochemie, Freie Universität Berlin, Spandauer Damm 130, D-1000, Berlin 19, Germany aut NATIONALLICENCE 700 1- Lohle W. Universitäts-HNO-Klinik der Universität Freiburg, Kilianstraße, D-7800, Freiburg, Germany aut NATIONALLICENCE 700 1- Karasiewicz Ch Institut für Klinische Chemie und Biochemie, Freie Universität Berlin, Spandauer Damm 130, D-1000, Berlin 19, Germany aut NATIONALLICENCE 700 1- Reutter W. Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33, Germany aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/3(1992-06-01), 132-140 0282-0080 9:3<132 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer