caa a22 4500 469115181 CHVBK 20180323133122.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00780761 doi (NATIONALLICENCE)springer-10.1007/BF00780761 Fine sugar specificity of the Butea frondosa seed lectin [Elektronische Daten] [Ahidjo Ayouba, Henry Debray, Pierre Rougé] Various monosaccharides and oligosaccharides were used to define the specificity of theButea frondosa lectin using the hapten inhibition technique of human erythrocyte agglutination. AlthoughB. frondosa lectin exhibited higher affinity forN-acetylgalactosamine, lactose andN-acetyllactosamine appeared to be relatively good inhibitors of haemagglutination. The behaviour ofN-acetyllactosamine-type oligosaccharides and glycopeptides on a column ofB. frondosa lectin immobilized on Sepharose 4B showed that the sugar-binding specificity of the lectin is directed towards unmaskedN-acetyllactosamine sequences. Substitution of theseN-acetyllactosamine sequences by sialic acid residues completely abolished the affinity of the lectin for the saccharides. The presence of one or several αFuc(1-3)GlcNAc groups completely inhibited the interaction between the glycopeptides and the lectin. Substitution of the core β-mannose residue by an additional bisecting β(1-4)GlcNAc residue decreases the affinity of the lectin for these structures as compared with the unsubstituted ones. Chapman & Hall, 1992 Butea frondosa lectin nationallicence sugar specificity nationallicence N -acetyllactosamine-type oligosaccharides nationallicence Ayouba Ahidjo Laboratoire de Biologie Cellulaire, Faculté des Sciences Pharmaceutiques, Université Paul Sabatier, 35 chemin des Maraîchers, 31062, Toulouse, France aut Debray Henry Laboratoire de Chimie Biologique, Unité Mixte de Recherche du CNRS no 111, Université des Sciences et Techniques de Lille-Flandre-Artois, 59655, Villeneuve d'Ascq, France aut Rougé Pierre Laboratoire de Biologie Cellulaire, Faculté des Sciences Pharmaceutiques, Université Paul Sabatier, 35 chemin des Maraîchers, 31062, Toulouse, France aut Glycoconjugate Journal Kluwer Academic Publishers 9/3(1992-06-01), 141-147 0282-0080 9:3<141 1992 9 10719 https://doi.org/10.1007/BF00780761 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00780761 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ayouba Ahidjo Laboratoire de Biologie Cellulaire, Faculté des Sciences Pharmaceutiques, Université Paul Sabatier, 35 chemin des Maraîchers, 31062, Toulouse, France aut NATIONALLICENCE 700 1- Debray Henry Laboratoire de Chimie Biologique, Unité Mixte de Recherche du CNRS no 111, Université des Sciences et Techniques de Lille-Flandre-Artois, 59655, Villeneuve d'Ascq, France aut NATIONALLICENCE 700 1- Rougé Pierre Laboratoire de Biologie Cellulaire, Faculté des Sciences Pharmaceutiques, Université Paul Sabatier, 35 chemin des Maraîchers, 31062, Toulouse, France aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/3(1992-06-01), 141-147 0282-0080 9:3<141 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer