caa a22 4500 46911519X CHVBK 20180323133122.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00731162 doi (NATIONALLICENCE)springer-10.1007/BF00731162 Ion-spray mass spectrometric analysis of glycosaminoglycan oligosaccharides [Elektronische Daten] [Keiichi Takagaki, Kaoru Kojima, Mitsuo Majima, Toshiya Nakamura, Ikunoshin Kato, Masahiko Endo] Oligosaccharides from hyaluronic acid and chondroitin 6-sulfate were prepared by digestion with testicular hyaluronidase and separated according to their degree of polymerization by gel-permeation chromatography. These materials were successively analyzed by negative-mode ion-spray mass spectrometry with an atmospheric-pressure ion source. An ion-spray interface was used to produce ions via the ion evaporation process, producing mass spectra containing a series of molecular species carrying multiple charges. Using two adjacent multiply charged molecular ions, the exact molecular weights up to the tetradecasaccharide were calculated with a precision of ±1 dalton. This type of mass spectrometry was also demonstrated to be feasible for the analysis of mixtures of oligosaccharides, including tetra-, hexa-, octa- and decasaccharides, from hyaluronic acid or chondroitin 6-sulfate without separation. Ion-spray mass spectrometry was thus shown to be applicable to the structural analysis of oligosaccharides from glycosaminoglycans. Chapman & Hall, 1992 Ion-spray mass spectrometry nationallicence glycosaminoglycans nationallicence hyaluronic acid nationallicence chondroitin 6-sulfate nationallicence HA : hyaluronic acid nationallicence Ch6S : chondroitin 6-sulfate nationallicence GAG : glycosaminoglycan nationallicence GlcA : d-glucuronic acid nationallicence GlcNAc : 2-acetamido-2-deoxy-d-glucose nationallicence GalNAc : 2-acetamido-2-deoxy-d-galactose nationallicence Takagaki Keiichi Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut Kojima Kaoru Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut Majima Mitsuo Kushiro Junior College, 1-10-42 Midorigaoka, 085, Kushiro, Japan aut Nakamura Toshiya Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut Kato Ikunoshin Research Institute for Glycotechnology, 82-4 Zaifu-cho, 036, Hirosaki, Japan aut Endo Masahiko Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut Glycoconjugate Journal Kluwer Academic Publishers 9/4(1992-08-01), 174-179 0282-0080 9:4<174 1992 9 10719 https://doi.org/10.1007/BF00731162 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00731162 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takagaki Keiichi Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut NATIONALLICENCE 700 1- Kojima Kaoru Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut NATIONALLICENCE 700 1- Majima Mitsuo Kushiro Junior College, 1-10-42 Midorigaoka, 085, Kushiro, Japan aut NATIONALLICENCE 700 1- Nakamura Toshiya Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut NATIONALLICENCE 700 1- Kato Ikunoshin Research Institute for Glycotechnology, 82-4 Zaifu-cho, 036, Hirosaki, Japan aut NATIONALLICENCE 700 1- Endo Masahiko Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/4(1992-08-01), 174-179 0282-0080 9:4<174 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer