caa a22 4500 469115262 CHVBK 20180323133122.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00731165 doi (NATIONALLICENCE)springer-10.1007/BF00731165 Surface glycoprotein of human natural killer cells recognized by wheat germ agglutinin [Elektronische Daten] [Kenji Harada, Hiroshi Yamane, Yasuyuki Imai, Tsutomu Tsuji, Satoshi Toyoshima, Toshiaki Osawa] We analyzed surface glycoproteins of human natural killer (NK) cells by utilizing lectins. Among the lectins tested, wheat germ agglutinin (WGA) was found to bind preferentially to CD16(Leu11)-positive lymphocytes as determined by two-colour flow cytometry. Analysis of glycoproteins in the lysate prepared from NK cells with sodium dodecyl sulfate (SDS) gel electrophoresis followed by Western blotting and125I labeled WGA staining revealed that a glycoprotein with anM r of 65 kDa was strongly bound to the lectin, but no corresponding glycoprotein was detected in the lysate of T lymphocytes. This glycoprotein (GP65) gave several spots in the pI range 4.1-4.6 on 2-dimensional gel electrophoresis. Sialidase treatment of GP65 resulted in a single spot on the 2-dimensional gel, suggesting that GP65 is heterogeneous in the degree of sialylation. GP65 was shown to be exposed on the cell surface, since it was radiolabeled with125I by the lactoperoxidase-catalyzed method. We next isolated GP65 from human peripheral blood lymphocytes by a combination of chromatography on a cation-exchange column and a WGA-agarose column and preparative SDS gel electrophoresis. It is suggested that GP65 is a novel surface glycoprotein on human NK cells. Chapman & Hall, 1992 Sialoglycoprotein nationallicence natural killer (NK) cells nationallicence wheat germ agglutinin nationallicence Harada Kenji Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan aut Yamane Hiroshi Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan aut Imai Yasuyuki Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan aut Tsuji Tsutomu Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan aut Toyoshima Satoshi Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan aut Osawa Toshiaki Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan aut Glycoconjugate Journal Kluwer Academic Publishers 9/4(1992-08-01), 198-203 0282-0080 9:4<198 1992 9 10719 https://doi.org/10.1007/BF00731165 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00731165 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harada Kenji Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Yamane Hiroshi Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Imai Yasuyuki Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Tsuji Tsutomu Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Toyoshima Satoshi Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Osawa Toshiaki Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/4(1992-08-01), 198-203 0282-0080 9:4<198 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer