caa a22 4500 469115297 CHVBK 20180323133123.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00731160 doi (NATIONALLICENCE)springer-10.1007/BF00731160 Use of resorufin-labelled N-glycopeptide in a high-performance liquid chromatography assay to monitor endoglycosidase activities during cultivation of Flavobacterium meningosepticum [Elektronische Daten] [Sylvain Bourgerie, Yannis Karamanos, Sylvie Berger, Raymond Julien] Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidase F (PNGase F) and endo-β-N-acetyl glucosaminidase F (Endo F) activities were monitored during cultivation ofFlavobacterium meningosepticum using a new fluorescence-HPLC procedure based on a commercially available substrate. The PNGase F activity reached a maximum level at the end of the log phase and remained constant during the stationary phase, while Endo F continuously increased until late stationary phase. PNGase F obtained at the end of the log phase was less contaminated by other proteins compared with late stationary phase. Chapman & Hall, 1992 Endoglycosidases assay nationallicence PNGase F nationallicence fluorescence-HPLC nationallicence Con A : concanavalin A nationallicence Endo F : endo-β-N-acetyl glucosaminidase F (EC 3.2.1.96) nationallicence GlcNAc : N-acetylglucosamine nationallicence PNGase F : peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidase F (EC 3.5.1.52) nationallicence Bourgerie Sylvain Institute de Biotechnologie, Université de Limoges, 123, avenue Albert Thomas, 87060, Limoges Cedex, France aut Karamanos Yannis Institute de Biotechnologie, Université de Limoges, 123, avenue Albert Thomas, 87060, Limoges Cedex, France aut Berger Sylvie Institute de Biotechnologie, Université de Limoges, 123, avenue Albert Thomas, 87060, Limoges Cedex, France aut Julien Raymond Institute de Biotechnologie, Université de Limoges, 123, avenue Albert Thomas, 87060, Limoges Cedex, France aut Glycoconjugate Journal Kluwer Academic Publishers 9/4(1992-08-01), 162-167 0282-0080 9:4<162 1992 9 10719 https://doi.org/10.1007/BF00731160 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00731160 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bourgerie Sylvain Institute de Biotechnologie, Université de Limoges, 123, avenue Albert Thomas, 87060, Limoges Cedex, France aut NATIONALLICENCE 700 1- Karamanos Yannis Institute de Biotechnologie, Université de Limoges, 123, avenue Albert Thomas, 87060, Limoges Cedex, France aut NATIONALLICENCE 700 1- Berger Sylvie Institute de Biotechnologie, Université de Limoges, 123, avenue Albert Thomas, 87060, Limoges Cedex, France aut NATIONALLICENCE 700 1- Julien Raymond Institute de Biotechnologie, Université de Limoges, 123, avenue Albert Thomas, 87060, Limoges Cedex, France aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/4(1992-08-01), 162-167 0282-0080 9:4<162 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer