caa a22 4500 469115319 CHVBK 20180323133123.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00731139 doi (NATIONALLICENCE)springer-10.1007/BF00731139 Comparison of the N -glycoloylneuraminic and N -acetylneuraminic acid content of platelets and their precursors using high performance anion exchange chromatography [Elektronische Daten] [T. Budd, C. Dolman, A. Lawson, W. Chai, J. Saxton, F. Hemming] N-Acetylneuraminic acid (Neu5Ac) andN-glycoloylneuraminic acid (Neu5Gc) are distributed widely in nature. Using a Carbopac PA-1 anion exchange column, we have determined the ratios of Neu5Ac and Neu5Gc in hydrolysates of platelets and their precursors: a rat promegakaryoblastic (RPM) cell line and a human megakaryoblastic leukemia cell line (MEG-01). The ratio of Neu5Gc:Neu5Ac in cultured RPM cells is 16:1, whereas in platelet rich plasma and cultured MEG-01 cells it is 1:38 and 1:28, respectively. The nature of these sialic acids from RPM cells was verified using thin layer chromatography and liquid secondary ion mass spectrometry. The relevance of increased Neu5Gc levels in early stages of development is discussed. Chapman & Hall, 1992 N -glycoloylneuraminic acid nationallicence N -acetylneuraminic acid nationallicence promegakaryoblast nationallicence platelet nationallicence high performance anion exchange chromatography nationallicence Neu5Ac : N-acetylneuraminic acid nationallicence Neu5Gc : N-glycoloylneuraminic acid nationallicence RPM : rat promegakaryoblast nationallicence MEG-01 : human megakaryoblastic leukaemia cell line nationallicence PAD : pulsed amperometric detection nationallicence WGA : wheat germ agglutinin nationallicence FCS : foetal calf serum nationallicence PPEADF : phosphatidylethanolamine dipalmitoyl nationallicence LSIMS : liquid secondary ion mass spectrometry nationallicence HPAEC : high performance anion exchange chromatography nationallicence TBA : thiobarbituric acid nationallicence Budd T. Department of Biochemistry, Medical School, Queen's Medical Centre, NG7 2UH, Nottingham, UK aut Dolman C. Department of Biochemistry, Medical School, Queen's Medical Centre, NG7 2UH, Nottingham, UK aut Lawson A. Section of Clinical Mass Spectrometry, MRC Clinical Research Centre, Watford Road, HA1 3UJ, Harrow, Middlesex, UK aut Chai W. Section of Clinical Mass Spectrometry, MRC Clinical Research Centre, Watford Road, HA1 3UJ, Harrow, Middlesex, UK aut Saxton J. Department of Biochemistry, Medical School, Queen's Medical Centre, NG7 2UH, Nottingham, UK aut Hemming F. Department of Biochemistry, Medical School, Queen's Medical Centre, NG7 2UH, Nottingham, UK aut Glycoconjugate Journal Kluwer Academic Publishers 9/5(1992-10-01), 274-278 0282-0080 9:5<274 1992 9 10719 https://doi.org/10.1007/BF00731139 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00731139 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Budd T. Department of Biochemistry, Medical School, Queen's Medical Centre, NG7 2UH, Nottingham, UK aut NATIONALLICENCE 700 1- Dolman C. Department of Biochemistry, Medical School, Queen's Medical Centre, NG7 2UH, Nottingham, UK aut NATIONALLICENCE 700 1- Lawson A. Section of Clinical Mass Spectrometry, MRC Clinical Research Centre, Watford Road, HA1 3UJ, Harrow, Middlesex, UK aut NATIONALLICENCE 700 1- Chai W. Section of Clinical Mass Spectrometry, MRC Clinical Research Centre, Watford Road, HA1 3UJ, Harrow, Middlesex, UK aut NATIONALLICENCE 700 1- Saxton J. Department of Biochemistry, Medical School, Queen's Medical Centre, NG7 2UH, Nottingham, UK aut NATIONALLICENCE 700 1- Hemming F. Department of Biochemistry, Medical School, Queen's Medical Centre, NG7 2UH, Nottingham, UK aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/5(1992-10-01), 274-278 0282-0080 9:5<274 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer