caa a22 4500 469115335 CHVBK 20180323133123.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00731134 doi (NATIONALLICENCE)springer-10.1007/BF00731134 Novel N -linked oligo-mannose type oligosaccharides containing an α- d -galactofuranosyl linkage found in α- d -galactosidase from Aspergillus niger [Elektronische Daten] [Tsutomu Takayanagi, Katsuhiko Kushida, Kyoko Idonuma, Katsumi Ajisaka] Structures of oligosaccharides fromAspergillus niger α-d-galactosidase [EC 3.2.1.22] were studied. Purified α-d-galactosidase was treated withN-glycosidase F, and six kinds of oligosaccharides were isolated by gel chromatography and anion-exchange chromatography. The structures of the oligosaccharides were determined by1H-NMR and compositional analysis to be Man5GlcNAc2, Man6GlcNAc2, Man9GlcNAc2, GlcMan9GlcNAc2, GalMan4GlcNAc2 and GalMan5GlcNAc2. From mild acid hydrolysis, methylation analysis and ROESY spectral analysis, it was ascertained that the galactosyl residue in two oligosaccharides was in the furanose form and was bound to mannose at the nonreducing end with an α1-2 linkage (GalfMan4GlcNAc2 and GalfMan5GlcNAc2). Chapman & Hall, 1992 α- d -galactosidase nationallicence N -linked oligosaccharide nationallicence α- d -galactofuranosyl linkage nationallicence Aspergillus niger nationallicence Takayanagi Tsutomu Meiji Institute of Health Science, Meiji Milk Products Co. Ltd., 540 Naruda, 250, Odawara, Japan aut Kushida Katsuhiko Varian Instruments Ltd., 2-2-6 Ohkubo, Shinjuku-ku, 169, Tokyo, Japan aut Idonuma Kyoko Meiji Institute of Health Science, Meiji Milk Products Co. Ltd., 540 Naruda, 250, Odawara, Japan aut Ajisaka Katsumi Meiji Institute of Health Science, Meiji Milk Products Co. Ltd., 540 Naruda, 250, Odawara, Japan aut Glycoconjugate Journal Kluwer Academic Publishers 9/5(1992-10-01), 229-234 0282-0080 9:5<229 1992 9 10719 https://doi.org/10.1007/BF00731134 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00731134 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takayanagi Tsutomu Meiji Institute of Health Science, Meiji Milk Products Co. Ltd., 540 Naruda, 250, Odawara, Japan aut NATIONALLICENCE 700 1- Kushida Katsuhiko Varian Instruments Ltd., 2-2-6 Ohkubo, Shinjuku-ku, 169, Tokyo, Japan aut NATIONALLICENCE 700 1- Idonuma Kyoko Meiji Institute of Health Science, Meiji Milk Products Co. Ltd., 540 Naruda, 250, Odawara, Japan aut NATIONALLICENCE 700 1- Ajisaka Katsumi Meiji Institute of Health Science, Meiji Milk Products Co. Ltd., 540 Naruda, 250, Odawara, Japan aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/5(1992-10-01), 229-234 0282-0080 9:5<229 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer