caa a22 4500 46911536X CHVBK 20180323133123.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00731138 doi (NATIONALLICENCE)springer-10.1007/BF00731138 Ganglioside alterations in YAC-1 cells cultivated in serum-supplemented and serum-free growth medium [Elektronische Daten] [Johannes Müthing, Antje Pörtner, Volker Jäger] Gangliosides of the ‘GM1b-pathway' (GM1b and GalNAc-GM1b) have been found to be highly expressed by the mouse T lymphoma YAC-1 grown in serum-supplemented medium, whereas GM2 and GM1 (‘GM1a-pathway') occurred only in low amounts [Müthing, J., Peter-Katalinić, J., Hanisch, F.-G., Neumann, U. (1991)Glycoconjugate J 8:414-23]. Considerable differences in the ganglioside composition of YAC-1 cells grown in serum-supplemented and in well defined serum-free medium were observed. After transfer of the cells from serum-supplemented medium (RPMI 1640 with 10% fetal calf serum) to serum-free medium (RPMI 1640 with well defined supplements), GM1b and GalNAc-GM1b decreased and only low amounts of these gangliosides could be detected in serum-free growing cells. The expression of GM1a was also diminished but not as strongly as that of GM1b and GalNAc-GM1b. These growth medium mediated ganglioside alterations were reversible, and the original ganglioside expression was achieved by readaptation of serum-free growing cells to the initial serum-supplemented medium. On the other hand, a ‘new' ganglioside, supposed to represent GalNAc-GD1a and not expressed by serum-supplemented growing cells, was induced during serum-free cultivation, and increased strongly after readaptation. These observations reveal that the ganglioside composition ofin vitro cultivated cells can be modified by the extracellular environment due to different supplementation of the basal growth medium. Chapman & Hall, 1992 YAC-1 T lymphoma nationallicence GM1b-type gangliosides nationallicence antibodies nationallicence choleragenoid nationallicence overlay technique nationallicence cell cultivation nationallicence serum nationallicence Müthing Johannes Institut für Zellkulturtechnik, Technische Fakultät, Universität Bielefeld, Bielefeld, Germany aut Pörtner Antje Institut für Zellkulturtechnik, Technische Fakultät, Universität Bielefeld, Bielefeld, Germany aut Jäger Volker Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany aut Glycoconjugate Journal Kluwer Academic Publishers 9/5(1992-10-01), 265-273 0282-0080 9:5<265 1992 9 10719 https://doi.org/10.1007/BF00731138 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00731138 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Müthing Johannes Institut für Zellkulturtechnik, Technische Fakultät, Universität Bielefeld, Bielefeld, Germany aut NATIONALLICENCE 700 1- Pörtner Antje Institut für Zellkulturtechnik, Technische Fakultät, Universität Bielefeld, Bielefeld, Germany aut NATIONALLICENCE 700 1- Jäger Volker Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/5(1992-10-01), 265-273 0282-0080 9:5<265 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer