caa a22 4500 469115386 CHVBK 20180323133123.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00731133 doi (NATIONALLICENCE)springer-10.1007/BF00731133 Synthesis of methyl α- and β- N -dansyl- d -galactosaminides, probes for the combining sites of N -acetyl- d -galactosamine-specific lectins [Elektronische Daten] [Willy Kinzy, David Belenky, Frank Loontiens, Nathan Sharon] The synthesis of the methyl α- and β-N-dansyl-d-galactosaminides is described using methyl α,β-2-azido-2-deoxy-d-galactopyranoside as starting material. This was reduced to the corresponding methyl α,β-2-amino-2-deoxy-d-galactopyranoside and then treated with dansyl chloride to yield a mixture of methyl α,β-N-dansyl-d-galactosaminides which was separated into individual anomeric forms by flash chromatography on silica gel. Methyl α-N-dansyl-d-galactosaminide was used as a fluorescent indicator ligand in continuous substitution titrations to determine the association constants of nonchromophoric carbohydrates with theN-acetyl-d-galactosamine specific lectin fromErythrina corallodendron. Chapman & Hall, 1992 N -Acetylgalactosamine nationallicence lectins nationallicence dansyl derivatives nationallicence spectrofluorimetry nationallicence association constants nationallicence ECorL : Erythrina corallodendron lectin nationallicence MeαGalNDns : methyl 2-deoxy-2-(5-dimethylamino-1-naphthalenesulfamido)-α-d-galactopyranoside nationallicence MeβGalNDns : methyl 2-deoxy-2-(5-dimethylamino-1-naphthalenesulfamido)-β-d-galactopyranoside nationallicence Kinzy Willy Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot, Israel aut Belenky David Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot, Israel aut Loontiens Frank Laboratory of Biochemistry, Faculty of Science, State University of Gent, B-9000, Gent, Belgium aut Sharon Nathan Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot, Israel aut Glycoconjugate Journal Kluwer Academic Publishers 9/5(1992-10-01), 225-227 0282-0080 9:5<225 1992 9 10719 https://doi.org/10.1007/BF00731133 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00731133 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kinzy Willy Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot, Israel aut NATIONALLICENCE 700 1- Belenky David Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot, Israel aut NATIONALLICENCE 700 1- Loontiens Frank Laboratory of Biochemistry, Faculty of Science, State University of Gent, B-9000, Gent, Belgium aut NATIONALLICENCE 700 1- Sharon Nathan Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot, Israel aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/5(1992-10-01), 225-227 0282-0080 9:5<225 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer