caa a22 4500 469115408 CHVBK 20180323133123.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00731705 doi (NATIONALLICENCE)springer-10.1007/BF00731705 Petryniak Jerzy Department of Biological Chemistry, University of Michigan, 48109, Ann Arbor, Michigan, USA aut α- d -Galactosylation of surface fucoglycoconjugate(s) upon stimulation/activation of murine peritoneal macrophages [Elektronische Daten] [Jerzy Petryniak] Murine resident macrophages express, on their surface, carbohydrate epitopes which undergo changes during their stimulation/activation as monitored by binding of125I labelledEvonymus europaea andGriffonia simplicifolia I-B4 lectins. Treatment of the stimulated macrophages with coffee bean α-galactosidase abolished binding of the GS I-B4 isolectin and changed the binding pattern of theEvonymus lectin. The affinity (K a) ofEvonymus lectin for α-galactosidase-treated macrophages decreased approximately 23-fold, from 1.25×108 M−1 to 5.5×106 M−1. Subsequent digestion of α-galactosidase-treated macrophages with α-l-fucosidase fromTrichomonas foetus, further reduced binding ofEvonymus lectin. Resident macrophages showed the same pattern ofEvonymus lectin binding, with the same affinity, as α-galactosidase-treated, stimulated macrophages. These results, together with a consideration of the carbohydrate binding specificity of theEvonymus lectin which, in the absence of α-d-galactosyl groups, requires α-l-fucosyl groups for binding, indicate the presence, on resident macrophages, of glycoconjugates with terminal α-l-fucosyl residues. It is also concluded that during macrophage stimulation/activation α-d-galactosyl residues are added to this glycoconjugate and that they form part of the receptor forEvonymus lectin. The same glycoconjugate(s) is/are also expressed on the activated macrophage IC-21 cell line which exhibits the same characteristics as that of stimulated peritoneal macrophages, i.e., it contains α-d-galactosyl end groups and is resistant to the action of trypsin. Both lectins were also specifically bound toCorynaebacterium parvum activated macrophages. Chapman & Hall, 1992 Macrophage nationallicence fucoglycoconjugate nationallicence galactosylation nationallicence BSA : bovine serum albumin nationallicence GS I-B4 : Griffonia simplicifolia I-B4 isolectin nationallicence PBS : 0.01m phosphate buffer (pH 7.1) with 0.15m NaCl (unless stated otherwise this buffer contained 3mm azide and was free of divalent cations) nationallicence PMSF : phenyl methane sulfonyl fluoride nationallicence TG : thioglycollate brewers medium nationallicence Glycoconjugate Journal Kluwer Academic Publishers 9/2(1992-04-01), 92-98 0282-0080 9:2<92 1992 9 10719 https://doi.org/10.1007/BF00731705 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00731705 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Petryniak Jerzy Department of Biological Chemistry, University of Michigan, 48109, Ann Arbor, Michigan, USA aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/2(1992-04-01), 92-98 0282-0080 9:2<92 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer