caa a22 4500 469115467 CHVBK 20180323133123.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00731703 doi (NATIONALLICENCE)springer-10.1007/BF00731703 α1-6(α1-3)-Difucosylation of the asparagine-bound N -acetylglucosamine in honeybee venom phospholipase A2 [Elektronische Daten] [Erika Staudacher, Friedrich Altmann, Leopold März, Karl Hård, Johannis Kamerling, Johannes Vliegenthart] Chymotryptic glycopeptides were prepared from a honeybee (Apis mellifica) venom phospholipase A2 (E.C. 3.1.1.4) fraction, with high affinity towards lentil (Lens culinaris) lectin. Treatment of the glycopeptide mixture with peptide-N 4-(N-acetyl-β-glucosaminyl)asparagine amidase A, followed by HPLC fractionation, yielded two oligosaccharides, which were analysed by 500 MHz1H-NMR spectroscopy to give the following structures $$\begin{array}{*{20}c} {Man\alpha 1} & \backslash & {Fuc\alpha 1} & | \\ {} & 6 & {Man\beta 1 - 4GlcNAc\beta 1 - 4GlcNAc} & 6 \\ {} & 3 & {} & {} \\ {Man\alpha 1} & / & {} & {} \\ \end{array} \begin{array}{*{20}c} {Man\alpha 1} & \backslash & {Fuc\alpha 1} & | \\ {} & 6 & {Man\beta 1 - 4GlcNAc\beta 1 - 4GlcNAc} & 6 \\ {} & 3 & 3 & | \\ {Man\alpha 1} & / & {Fuc\alpha 1} & {} \\ \end{array}$$ This is the first report on a naturally occurring glycoprotein N-glycan with two fucose residues linked to the asparagine-boundN-acetylglucosamine. Chapman & Hall, 1992 Insect glycoprotein nationallicence N-glycan nationallicence fucosylation nationallicence Fuc : fucose nationallicence PLA2 : phospholipase A2 nationallicence PNGase A : peptide-N 4-(N-acetyl-β-glucosaminyl)asparagine amidase A nationallicence PNGase F : peptide-N 4-(N-acetyl-β-glucosaminyl)asparagine amidase F nationallicence TLCK : Nα-p-tosyl-l-lysine-chloromethylketone nationallicence 2D HOHAHA : 2-dimensional homonuclear Hartmann-Hahn nationallicence Staudacher Erika Institut für Chemie, Universität für Bodenkultur, A-1180, Vienna, Austria aut Altmann Friedrich Institut für Chemie, Universität für Bodenkultur, A-1180, Vienna, Austria aut März Leopold Institut für Chemie, Universität für Bodenkultur, A-1180, Vienna, Austria aut Hård Karl Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, NL-3584 CH, Utrecht, The Netherlands aut Kamerling Johannis Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, NL-3584 CH, Utrecht, The Netherlands aut Vliegenthart Johannes Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, NL-3584 CH, Utrecht, The Netherlands aut Glycoconjugate Journal Kluwer Academic Publishers 9/2(1992-04-01), 82-85 0282-0080 9:2<82 1992 9 10719 https://doi.org/10.1007/BF00731703 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00731703 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Staudacher Erika Institut für Chemie, Universität für Bodenkultur, A-1180, Vienna, Austria aut NATIONALLICENCE 700 1- Altmann Friedrich Institut für Chemie, Universität für Bodenkultur, A-1180, Vienna, Austria aut NATIONALLICENCE 700 1- März Leopold Institut für Chemie, Universität für Bodenkultur, A-1180, Vienna, Austria aut NATIONALLICENCE 700 1- Hård Karl Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, NL-3584 CH, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Kamerling Johannis Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, NL-3584 CH, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Vliegenthart Johannes Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, NL-3584 CH, Utrecht, The Netherlands aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/2(1992-04-01), 82-85 0282-0080 9:2<82 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer