caa a22 4500 469115483 CHVBK 20180323133123.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00731701 doi (NATIONALLICENCE)springer-10.1007/BF00731701 Preparation of N -acetylneuraminic acid from delipidated egg yolk [Elektronische Daten] [Mamoru Koketsu, Lekh Juneja, Hiroshi Kawanami, Mujo Kim, Takehiko Yamamoto] Egg yolk, a large proportion of the egg, was studied for the preparation ofN-acetylneuraminic acid (Neu5Ac). The delipidated hen egg yolk (DEY; 500 kg containing 0.2% w/w, Neu5Ac) was hydrolysed with HCl (pH 1.4) at 80 °C and neutralized with NaOH (pH 6.0). The mixture was filtered and electrodialysed until the conductivity was 240 µS cm−1. The filtrate was applied on a column of Dowex HCR-W2 (20-50 mesh), followed by a column of Dowex 1-X8 (200-400 mesh). The latter column was washed with water, and then eluted with a linear gradient of HCO2H (0-2m). The eluates containing Neu5Ac were concentrated using a reverse osmosis membrane and, finally, rotary evaporated at 40 °C. The residue was then lyophilized to yield 500 g Neu5Ac. The purity of Neu5Ac was >98% (TBA method). HPLC, NMR spectroscopy and TLC chromatography of the product obtained from the DEY showed that Neu5Ac was the sole derivative present in egg yolk. The DEY, a byproduct from egg processing plants, was found to be an excellent source for the large-scale preparation of Neu5Ac. Chapman & Hall, 1992 sialic acid nationallicence N -acetylneuraminic acid nationallicence delipidation nationallicence egg yolk nationallicence electrodialysis nationallicence Neu5Ac : N-acetylneuraminic acid nationallicence Neu5Gc : N-glycolylneuraminic acid nationallicence DEY : delipidated egg yolk nationallicence HPLC : high performance liquid chromatography nationallicence TLC : thin layer chromatography nationallicence NMR : nuclear magnetic resonance nationallicence IR : infrared spectroscopy nationallicence Koketsu Mamoru Basic Research Laboratories, Taiyo Kagaku Co. Ltd, 1-3 Takaramachi, 510 (Mie), Yokkaichi, Japan aut Juneja Lekh Basic Research Laboratories, Taiyo Kagaku Co. Ltd, 1-3 Takaramachi, 510 (Mie), Yokkaichi, Japan aut Kawanami Hiroshi Basic Research Laboratories, Taiyo Kagaku Co. Ltd, 1-3 Takaramachi, 510 (Mie), Yokkaichi, Japan aut Kim Mujo Basic Research Laboratories, Taiyo Kagaku Co. Ltd, 1-3 Takaramachi, 510 (Mie), Yokkaichi, Japan aut Yamamoto Takehiko Department of Biotechnology, Fukuyama University, Fukuyama, 729-02, Hiroshima, Japan aut Glycoconjugate Journal Kluwer Academic Publishers 9/2(1992-04-01), 70-74 0282-0080 9:2<70 1992 9 10719 https://doi.org/10.1007/BF00731701 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00731701 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koketsu Mamoru Basic Research Laboratories, Taiyo Kagaku Co. Ltd, 1-3 Takaramachi, 510 (Mie), Yokkaichi, Japan aut NATIONALLICENCE 700 1- Juneja Lekh Basic Research Laboratories, Taiyo Kagaku Co. Ltd, 1-3 Takaramachi, 510 (Mie), Yokkaichi, Japan aut NATIONALLICENCE 700 1- Kawanami Hiroshi Basic Research Laboratories, Taiyo Kagaku Co. Ltd, 1-3 Takaramachi, 510 (Mie), Yokkaichi, Japan aut NATIONALLICENCE 700 1- Kim Mujo Basic Research Laboratories, Taiyo Kagaku Co. Ltd, 1-3 Takaramachi, 510 (Mie), Yokkaichi, Japan aut NATIONALLICENCE 700 1- Yamamoto Takehiko Department of Biotechnology, Fukuyama University, Fukuyama, 729-02, Hiroshima, Japan aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/2(1992-04-01), 70-74 0282-0080 9:2<70 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer