caa a22 4500 469116064 CHVBK 20180323133125.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02755885 doi (NATIONALLICENCE)springer-10.1007/BF02755885 Elevation of intestinal substance P by acute acetic acid in rabbits: Modification by infant formulas [Elektronische Daten] [M. Miller, M. Becerra, M. Wong, X. Zhang, H. Sadowska-Krowicka, D. Albrecht, D. Clark, A. Jeng] To determine if infant formulas affect the enteric neuronal response to gut injury, we quantified, in anaesthetized rabbits, the time-dependent increase in intestinal substance P (SP) content in response to luminal acetic acid alone and in combination with various infant formulas. Loops of distal small intestine received by transmural injection, either saline or acetic acid (200 mmol/L) + calcium gluconate (50 mg/ml), alone or in combination with three infant formulas which differed in protein and lipid composition. Intestinal SP was extracted in boiling HCl (0.1 mol/L); levels were quantified by radioimmunoassay in a double blind manner. Acetic acid treatment for 45 min did not affect SP content; however, a significant increase (24-47%) was observed after 3 h (p<0.05). This increase in SP content was abolished by co-administration with a whey-hydrolysate infant formula (p<0.01), attenuated by a whey-based formula (p<0.05) and unaltered by a casein-predominant formula. Peptide preparations (hydrolysates) of casein or whey did not modify the intestinal SP response to acetic acid. However, casein hydrolysate, and, to a lesser extent, whey hydrolysate (p<0.05), potentiated the contractile effects of SP and bradykinin in guinea-pig ileum. We conclude that milk-derived factors modify the neurogenic response to acute gut injury. Infant formulas which better approximate the lipid and protein composition of breast milk may modulate the mucosal response to luminal irritants. Kluwer Acedemic Publishers, 1992 substance P nationallicence bradykinin nationallicence casein nationallicence whey nationallicence inflammation nationallicence peptidase nationallicence necrotizing enterocolitis nationallicence milk nationallicence Miller M. Department of Pediatrics, Louisiana State University Medical Center, 1542 Tulane Ave., 70112-2822, New Orleans, LA, USA aut Becerra M. Department of Pediatrics, Louisiana State University Medical Center, 1542 Tulane Ave., 70112-2822, New Orleans, LA, USA aut Wong M. Research Department Pharmaceuticals Division, Ciba-Geigy Corporation, Summit, New Jersey, USA aut Zhang X. Department of Pediatrics, Louisiana State University Medical Center, 1542 Tulane Ave., 70112-2822, New Orleans, LA, USA aut Sadowska-Krowicka H. Department of Pediatrics, Louisiana State University Medical Center, 1542 Tulane Ave., 70112-2822, New Orleans, LA, USA aut Albrecht D. Department of Pediatrics, Louisiana State University Medical Center, 1542 Tulane Ave., 70112-2822, New Orleans, LA, USA aut Clark D. Department of Pediatrics, Louisiana State University Medical Center, 1542 Tulane Ave., 70112-2822, New Orleans, LA, USA aut Jeng A. Research Department Pharmaceuticals Division, Ciba-Geigy Corporation, Summit, New Jersey, USA aut InflammoPharmacology Birkhäuser-Verlag 1/4(1992-12-01), 353-362 0925-4692 1:4<353 1992 1 10787 https://doi.org/10.1007/BF02755885 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02755885 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miller M. Department of Pediatrics, Louisiana State University Medical Center, 1542 Tulane Ave., 70112-2822, New Orleans, LA, USA aut NATIONALLICENCE 700 1- Becerra M. Department of Pediatrics, Louisiana State University Medical Center, 1542 Tulane Ave., 70112-2822, New Orleans, LA, USA aut NATIONALLICENCE 700 1- Wong M. Research Department Pharmaceuticals Division, Ciba-Geigy Corporation, Summit, New Jersey, USA aut NATIONALLICENCE 700 1- Zhang X. Department of Pediatrics, Louisiana State University Medical Center, 1542 Tulane Ave., 70112-2822, New Orleans, LA, USA aut NATIONALLICENCE 700 1- Sadowska-Krowicka H. Department of Pediatrics, Louisiana State University Medical Center, 1542 Tulane Ave., 70112-2822, New Orleans, LA, USA aut NATIONALLICENCE 700 1- Albrecht D. Department of Pediatrics, Louisiana State University Medical Center, 1542 Tulane Ave., 70112-2822, New Orleans, LA, USA aut NATIONALLICENCE 700 1- Clark D. Department of Pediatrics, Louisiana State University Medical Center, 1542 Tulane Ave., 70112-2822, New Orleans, LA, USA aut NATIONALLICENCE 700 1- Jeng A. Research Department Pharmaceuticals Division, Ciba-Geigy Corporation, Summit, New Jersey, USA aut NATIONALLICENCE 773 0- InflammoPharmacology Birkhäuser-Verlag 1/4(1992-12-01), 353-362 0925-4692 1:4<353 1992 1 10787 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer