caa a22 4500 469116153 CHVBK 20180323133125.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00172453 doi (NATIONALLICENCE)springer-10.1007/BF00172453 Mies Günter Max-Planck-Institute for Neurological Research, Department of Experimental Neurology, Gleueler Strafe 50, Cologne, Germany aut Measurement of in vivo glucose transport from blood to tissue of experimentally-induced glioma in rat brain [Elektronische Daten] [Günter Mies] Summary: In experimentally-induced F98 glioma of rat brain, regional blood flow and glucose transfer were assessed by means of double tracer autoradiography to measure Michaelis-Menten constants for the determination of unidirectional glucose transport across the blood-tumor and blood-brain barrier. In brain regions opposite the tumor hemisphere, the maximal glucose transport rate constant, Tm, ranged from 1.41 ± 0.12 to 3.22 ± 0.29 μmol/g/min and the half saturation transport constant of glucose, Kt, varied from 2.78 ± 0.83 to 5.6 ± 1.94 μmol/ml (estimate ± standard error of the estimate) yielding a normoglycemic unidirectional glucose inward transport which ranged from 1.24 ± 0.24 to 1.97 ± 0.13 μmol/g/min (mean ± standard deviation). In the tumor periphery, the Tm and the Kt values were 3.64 ± 0.56 μmol/g/ml and 7.32 ± 2.12 μmol/min, and in the tumor center, 1.77 ± 0.25 μmol/ml and 2.76 ± 1.13 μmol/min, respectively. The unidirectional glucose influx of tumor periphery and center in normoglycemia was 1.98 ± 0.22 and 1.34 ± 0.16 μmol/g/min, respectively. Despite comparable unidirectional glucose influxes, however, glucose metabolism of tumor tissue located in the periphery (0.83 ± 0.12 μmol/g/min) and the center (0.41 ± 0.10 μmol/g/min) of the tumor mass exceeded that of normal gray matter by about 68% and 100% which indicates uncoupling between glucose transport and phosphorylation in experimentally-induced F98 glioma of rat brain. Kluwer Academic Publishers, 1992 tumors nationallicence double tracer autoradiography nationallicence cerebral blood flow nationallicence glucose transport nationallicence glucose metabolism nationallicence Journal of Neuro-Oncology Kluwer Academic Publishers 12/1(1992-01-01), 13-23 0167-594X 12:1<13 1992 12 11060 https://doi.org/10.1007/BF00172453 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172453 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Mies Günter Max-Planck-Institute for Neurological Research, Department of Experimental Neurology, Gleueler Strafe 50, Cologne, Germany aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 12/1(1992-01-01), 13-23 0167-594X 12:1<13 1992 12 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer