caa a22 4500 469116234 CHVBK 20180323133125.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00172452 doi (NATIONALLICENCE)springer-10.1007/BF00172452 Monoclonal antibody against ependymoma-derived cell line [Elektronische Daten] [Yosuke Mihara, Yasuhiko Matsukado, Satoshi Goto, Yukitaka Ushio, Shin-ichi Tokumitsu, Kiyoshi Takahashi] Summary: Mouse myeloma cells were fused with spleen cells from mice that had been immunized with a human ependymoma derived cell line, KMS II. Hybridomas producing monoclonal antibodies (MAbs) were screened and cloned. Specificity of the antibody was determined by enzyme-linked immunosorbent assay (ELISA) and/or indirect immunofluorescence assay. One of the MAbs, designated Ep-C4 (subclass = IgGl), reacted with two cell lines derived from ependymoma but did not react with 17 cell lines derived from other types of brain tumor nor with 4 neuroblastoma cell lines or 19 cell lines derived from carcinoma, hematopoietic tumors and amnion. Indirect immunofluorescence and immuno-electron microscopy studies revealed that the antigen recognized by MAb Ep-C4 was located on cell surface membrane. The membrane antigen of KMS II cells, immunoprecipitated by MAb Ep-C4, was a protein of 81,000 dalton. The reactivity of MAb Ep-C4 was further examined using immunofluorescence and/or immunoperoxidase methods and frozen sections and short-term cultures of various types of brain tumors. No cross-reactivity with normal adult or fetal brain tissues was detected by absorption assay and immunoperoxidase staining. Our results suggest that the antigen defined by MAb Ep-C4 is specific for ependymoma cells, and different from the antigens of glioma cells or other neuroectodermal-derived cells previously described. Kluwer Academic Publishers, 1992 monoclonal antibody nationallicence ependymoma nationallicence glioma cell lines nationallicence enzyme-linked immunosorbent assay nationallicence Mihara Yosuke Department of Neurosurgery, Kumamoto University Medical School, 860, Kumamoto, Japan aut Matsukado Yasuhiko Department of Neurosurgery, Kumamoto University Medical School, 860, Kumamoto, Japan aut Goto Satoshi Department of Neurosurgery, Kumamoto University Medical School, 860, Kumamoto, Japan aut Ushio Yukitaka Department of Neurosurgery, Kumamoto University Medical School, 860, Kumamoto, Japan aut Tokumitsu Shin-ichi Department of Pathology, Kumamoto University Medical School, 860, Kumamoto, Japan aut Takahashi Kiyoshi Department of Pathology, Kumamoto University Medical School, 860, Kumamoto, Japan aut Journal of Neuro-Oncology Kluwer Academic Publishers 12/1(1992-01-01), 1-11 0167-594X 12:1<1 1992 12 11060 https://doi.org/10.1007/BF00172452 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172452 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mihara Yosuke Department of Neurosurgery, Kumamoto University Medical School, 860, Kumamoto, Japan aut NATIONALLICENCE 700 1- Matsukado Yasuhiko Department of Neurosurgery, Kumamoto University Medical School, 860, Kumamoto, Japan aut NATIONALLICENCE 700 1- Goto Satoshi Department of Neurosurgery, Kumamoto University Medical School, 860, Kumamoto, Japan aut NATIONALLICENCE 700 1- Ushio Yukitaka Department of Neurosurgery, Kumamoto University Medical School, 860, Kumamoto, Japan aut NATIONALLICENCE 700 1- Tokumitsu Shin-ichi Department of Pathology, Kumamoto University Medical School, 860, Kumamoto, Japan aut NATIONALLICENCE 700 1- Takahashi Kiyoshi Department of Pathology, Kumamoto University Medical School, 860, Kumamoto, Japan aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 12/1(1992-01-01), 1-11 0167-594X 12:1<1 1992 12 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer