caa a22 4500 469116420 CHVBK 20180323133126.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00172658 doi (NATIONALLICENCE)springer-10.1007/BF00172658 Expression of LFA-1/ICAM-1 in CNS lymphomas: possible mechanism for lymphoma homing into the brain [Elektronische Daten] [Rifaat Bashir, Hugh Coakham, Fred Hochberg] We examined a possible role for the adhesion molecules LFA-1 and ICAM-1 in localizing central nervous system non-Hodgkin's lymphomas (CNS-NHLs) to the brain. Fresh frozen sections from 12 monoclonal CNS NHLs (11 primary, one secondary) were stained with monoclonal antibodies to LFA-1 α chain (CD11a), β chain (CD18) and, ICAM-1 (CD54). Additional staining made use of rat monoclonal antibodies to the human and mouse high endothelial venule antigens HECA 452 and MECA 79 and mouse ICAM-1. The expression of these same molecules was also studied in mice with severe combined immunodeficiency (SCID) mice, bearing intracranial human lymphoblastoid cells. Eleven of the CNS-NHL tumors expressed LFA-1α (one strongly, one intermediate, nine weakly). Nine of the tumors weakly expressed LFA-1β.. Nine of twelve tumors weakly expressed ICAM-1. In six of seven tumors definite blood vessels stained for ICAM-l. Non-tumor brain from two patients and non-tumor cerebral blood vessels showed no staining with CD11a, CD18 or CD54 antibodies. Strong expression of LFA-α and LFA-β as well as ICAM-1 was noted in human lymphoblastoid cells (LCLs)/SCID mouse CNS lymphomas. Tumor blood vessels in these mice stained for mouse ICAM-1. Normal SCID mouse brains showed no staining with CDIIα, CD18, CD54 or mouse ICAM-1 antibodies. Human, human/mouse CNS lymphomas, normal human, and mouse brains showed no staining with either HECA 452 or MECA 79. Our data suggests that CNS lymphomas express LFA-1α, LFA-1β and to a lesser degree ICAM-1 antigen, while tumor blood vessel endothelium expresses ICAM-1. LFA-1α-LFA-1β/ICAM-1 interaction may play a role in large cell lymphoma homing and persistence in the brain. Kluwer Academic Publishers, 1992 primary CNS lymphoma nationallicence non-Hodgkin's lymphoma nationallicence adhesion molecules nationallicence Bashir Rifaat Section of Neurology, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska aut Coakham Hugh Department of Neurosurgery, Frenchay Hospital, Bristol, United Kingdom aut Hochberg Fred Department of Neurology, Massachusetts General Hospital, Boston, Massachusetts, USA aut Journal of Neuro-Oncology Kluwer Academic Publishers 12/2(1992-02-01), 103-110 0167-594X 12:2<103 1992 12 11060 https://doi.org/10.1007/BF00172658 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172658 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bashir Rifaat Section of Neurology, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska aut NATIONALLICENCE 700 1- Coakham Hugh Department of Neurosurgery, Frenchay Hospital, Bristol, United Kingdom aut NATIONALLICENCE 700 1- Hochberg Fred Department of Neurology, Massachusetts General Hospital, Boston, Massachusetts, USA aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 12/2(1992-02-01), 103-110 0167-594X 12:2<103 1992 12 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer