caa a22 4500 469116714 CHVBK 20180323133126.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00172473 doi (NATIONALLICENCE)springer-10.1007/BF00172473 Fluorescent dye rhodamine 6G as a molecular probe to study drug resistance of C6 rat glioma cells [Elektronische Daten] [Yoshihito Matsumoto, Noboru Sasaoka, Takahiro Tsuchida, Takashi Fujiwara, Seigo Nagao, Takashi Ohmoto] Summary: A study was made of the membrane transport of cytoplasm and mitochondria stained fluorescence dye Rhodamine 6G (R6G). In rat glioma C6 cells and 1-(4-amino-2-metyl-5-pyrymidinyl)-metyl-3-(2-chloroetyl)-3-nitrosourea hydrochloride (ACNU) and vincristine (VCR) resistant cell lines (C6/ACNU, C6/VCR), the rate of uptake of R6G decreased in C6/VCR cells, but verapamil increased the intracellular accumulation of R6G in C6/VCR. The intracellular accumulation of R6G of C6/ACNU cells was essentially the same as that of wild-type cells. C6/ACNU cells did not show cross resistance and were sensitive to VCR and cisplatin. C6/VCR cells showed cross resistance to ACNU and CDDP, but C6/VCR cells in the presence of verapamil were more sensitive to drugs than C6/VCR cells in the absence of verapamil. We conclude that the reduction of R6G fluorescence staining intensity in C6/VCR cells compared to wild-type cells may be associated with the mechanism of multidrug resistance (MDR) but does not reflect the mechanism of resistance to ACNU. Verapamil increased the accumulation of R6G in C6/VCR cells and overcame MDR, suggesting that there is a correlation between the MDR overcoming effect and enhancement of R6G accumulation, and that this correlation validates the use of the R6G staining test for clinical and laboratory investigation of MDR. Kluwer Academic Publishers, 1992 Rhodamine 6G nationallicence multidrug resistance nationallicence P-glycoprotein nationallicence flow cytometer nationallicence glioma nationallicence verapamil nationallicence Matsumoto Yoshihito Department of Neurological Surgery, Kagawa Medical School, Kagawa, Japan aut Sasaoka Noboru Department of Neurological Surgery, Kagawa Medical School, Kagawa, Japan aut Tsuchida Takahiro Department of Neurological Surgery, Kagawa Medical School, Kagawa, Japan aut Fujiwara Takashi Department of Neurological Surgery, Kagawa Medical School, Kagawa, Japan aut Nagao Seigo Department of Neurological Surgery, Kagawa Medical School, Kagawa, Japan aut Ohmoto Takashi Department of Neurological Surgery, Okayama University, Okayama, Japan aut Journal of Neuro-Oncology Kluwer Academic Publishers 13/3(1992-07-01), 217-222 0167-594X 13:3<217 1992 13 11060 https://doi.org/10.1007/BF00172473 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172473 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsumoto Yoshihito Department of Neurological Surgery, Kagawa Medical School, Kagawa, Japan aut NATIONALLICENCE 700 1- Sasaoka Noboru Department of Neurological Surgery, Kagawa Medical School, Kagawa, Japan aut NATIONALLICENCE 700 1- Tsuchida Takahiro Department of Neurological Surgery, Kagawa Medical School, Kagawa, Japan aut NATIONALLICENCE 700 1- Fujiwara Takashi Department of Neurological Surgery, Kagawa Medical School, Kagawa, Japan aut NATIONALLICENCE 700 1- Nagao Seigo Department of Neurological Surgery, Kagawa Medical School, Kagawa, Japan aut NATIONALLICENCE 700 1- Ohmoto Takashi Department of Neurological Surgery, Okayama University, Okayama, Japan aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 13/3(1992-07-01), 217-222 0167-594X 13:3<217 1992 13 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer