caa a22 4500 469116838 CHVBK 20180323133127.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00172759 doi (NATIONALLICENCE)springer-10.1007/BF00172759 The 56 kd platelet-derived growth factor (PDGF)-related protein is phosphorylated and the most stable form in human glioma cells [Elektronische Daten] [Takaharu Nakamura, Iwao Takeshita, Takanori Inamura, Masashi Fukui] We report herein the presence of a 56 kd platelet derived growth factor (PDGF)-related protein as a phosphorylated form in human glioma cells. The phosphorylation of the 56 kd form was found to be the longest of all PDGF-related proteins. By Western blotting using a monoclonal anti-PDGF B-chain, the 80 kd, 56 kd, 40 kd, 28 kd and 17 kd PDGF-related proteins were detected, while after treatment among the nitrocellulose membrane transblotted cell extracts with alkaline phosphatase, 40 kd was the most densely observed while the 56 kd and 80 kd PDGF-related proteins were also detected. In a 32P flush labeling study, it was revealed that PDGF-related proteins incorporated with 32P were detected at 28, 32, 35, 40, 56 and 80 kd but the 17 kd monomer was not labeled. Among the labeled PDGF-related proteins, the 56 kd PDGF-related protein alone remained intracellularly for at least 16 hours. These results indicated that the PDGF-related proteins in human glioma cells are synthesized in a phosphorylated form and partly remain in a 56 kd phosphorylated form intracellularly. The 56 kd form may thus be the most stable form and likely has a substantial biological effect. Kluwer Academic Publishers, 1992 PDGF nationallicence phosphorylation nationallicence 56 kd nationallicence Nakamura Takaharu Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, 3-1-1, Maidashi, Higashiku, Fukuoka, Japan aut Takeshita Iwao Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, 3-1-1, Maidashi, Higashiku, Fukuoka, Japan aut Inamura Takanori Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, 3-1-1, Maidashi, Higashiku, Fukuoka, Japan aut Fukui Masashi Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, 3-1-1, Maidashi, Higashiku, Fukuoka, Japan aut Journal of Neuro-Oncology Kluwer Academic Publishers 13/2(1992-06-01), 105-109 0167-594X 13:2<105 1992 13 11060 https://doi.org/10.1007/BF00172759 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172759 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakamura Takaharu Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, 3-1-1, Maidashi, Higashiku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Takeshita Iwao Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, 3-1-1, Maidashi, Higashiku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Inamura Takanori Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, 3-1-1, Maidashi, Higashiku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Fukui Masashi Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University 60, 3-1-1, Maidashi, Higashiku, Fukuoka, Japan aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 13/2(1992-06-01), 105-109 0167-594X 13:2<105 1992 13 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer