caa a22 4500 469116994 CHVBK 20180323133127.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00172595 doi (NATIONALLICENCE)springer-10.1007/BF00172595 Modulation of BUdR labeling index in rat brain tumors following intracarotid ACNU administration [Elektronische Daten] [Katsuyoshi Mineura, Katsuo Watanabe, Ichiro Izumi, Masayoshi Kowada] Summary: Chloroethy1nitrosourea (CENU) chemotherapy has yielded limited benefit on survival of malignant brain tumors. Intracarotid administration of CENU is expected to have the advantage of increasing drug concentration reaching tumors. To understand basic knowledge of intracarotid chemotherapy, we monitor changes of proliferating rate after intracarotid injection of 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2chloroethyl)-3-nitrosourea hydrochloride (ACNU), using a bromodeoxyuridine (BUdR) labeling index (LI) in transplanted brain tumors of three cell strains. C6-2 tumor cells were in vitro sensitive to ACNU, and C6-2/ACNU and C6-1 cells were resistant. The drug sensitivity to ACNU was as follows: 11.9 μtM in the C6-2 cells, 46.0 μM in the C6-2/ACNU cells, and 49.7 μM in the C6-1 cells at SD10, which gives 10% survival of clonogenic cells. The intracarotid ACNU at a dose of 30 mg/kg abruptly decreased the LI to 11% (mean) from 36% in C6-2 transplanted tumors. The LI remained low between 12 and 48 hours after, and then increased to the pretreatment level by 96 hours. In contrast, the LI of C6-1 tumors transiently fell to 15% from 42% at 12 hours after the injection, and subsequently increased to 36% at 24 hours and 37% at 48 hours. These results indicate that intracarotid ACNU administration shortly suppresses proliferating activity of tumors and that combined and alternating chemotherapy are mandatory for enhancing effectiveness of brain tumor chemotherapy. Kluwer Academic Publishers, 1992 brain tumors nationallicence intracarotid administration nationallicence ACNU nationallicence BUdR labeling index nationallicence Mineura Katsuyoshi Neurosurgical Service, Akita University Hospital, Akita, Japan aut Watanabe Katsuo Neurosurgical Service, Akita University Hospital, Akita, Japan aut Izumi Ichiro Neurosurgical Service, Akita University Hospital, Akita, Japan aut Kowada Masayoshi Neurosurgical Service, Akita University Hospital, Akita, Japan aut Journal of Neuro-Oncology Kluwer Academic Publishers 14/3(1992-11-01), 201-205 0167-594X 14:3<201 1992 14 11060 https://doi.org/10.1007/BF00172595 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172595 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mineura Katsuyoshi Neurosurgical Service, Akita University Hospital, Akita, Japan aut NATIONALLICENCE 700 1- Watanabe Katsuo Neurosurgical Service, Akita University Hospital, Akita, Japan aut NATIONALLICENCE 700 1- Izumi Ichiro Neurosurgical Service, Akita University Hospital, Akita, Japan aut NATIONALLICENCE 700 1- Kowada Masayoshi Neurosurgical Service, Akita University Hospital, Akita, Japan aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 14/3(1992-11-01), 201-205 0167-594X 14:3<201 1992 14 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer