caa a22 4500 469117052 CHVBK 20180323133127.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00172598 doi (NATIONALLICENCE)springer-10.1007/BF00172598 Evaluation of blood-brain barrier permeability and the effect of interferon in mouse glioma model [Elektronische Daten] [Marzenna Wiranowska, Americo Gonzalvo, Samuel Saporta, Orlando Gonzalez, Leon Prockop] Summary: The aim of this study was to evaluate whether interferon [IFN] can affect intracerebrally grown glioma and how alteration of the blood-brain barrier [BBB] may influence this effect. An intracerebrally implanted glioma G-26 (G-26) mouse brain-tumor model was developed and used in these studies. Histological characterization of this intracerebrally grown tumor revealed its anaplastic character. The astrocytic origin of G-26 was evidenced by glial fibrillary acidic protein staining and electron microscopic visualization of glial filaments. A study of tumor progression and animal survival showed development of a well defined tumor nodule within approximately seven days after the implantation. The median animal survival time was 27 ±3.8 days. The integrity of the blood-brain barrier [BBB] within the tumor was evaluated by the intravenous injection of horseradish peroxidase at days 3, 7,10 and 20 after brain tumor implant and compared to ‘sham' controls. The tumor-induced BBB alteration was progressive from day 3 to day 20. Glioma-26 subcutaneously passed in C57BL/6 mice was also continously cultured in vitro. Its proliferation was inhibited by homologous mouse interferon α/β [MuIFN α/β but not by human interferon a lymphoblastoid or human interferon ß. The in vivo studies of G-26 glioma treatment with MuIFN α/β were performed using single bolus of IFN in osmotically altered animals or slow IFN infusion through osmotic micro-pumps. The slow infusion of IFN had no effect on animal survival. However, a statistically significant increase in animal survival was observed after single bolus IFN treatment following osmotic BBB alteration. Kluwer Academic Publishers, 1992 mouse glioma nationallicence mouse interferon α/ß (MuIFN a/ß) nationallicence intracerebral brain tumor model nationallicence immunotherapy nationallicence blood-brain barrier (BBB) nationallicence Wiranowska Marzenna Department of Neurology, University of South Florida, College of Medicine, Tampa, Florida, USA aut Gonzalvo Americo Department of Pathology, University of South Florida, College of Medicine, Tampa, Florida, USA aut Saporta Samuel Department of Anatomy, University of South Florida, College of Medicine, Tampa, Florida, USA aut Gonzalez Orlando Department of Pathology, University of South Florida, College of Medicine, Tampa, Florida, USA aut Prockop Leon Department of Neurology, University of South Florida, College of Medicine, Tampa, Florida, USA aut Journal of Neuro-Oncology Kluwer Academic Publishers 14/3(1992-11-01), 225-236 0167-594X 14:3<225 1992 14 11060 https://doi.org/10.1007/BF00172598 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172598 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wiranowska Marzenna Department of Neurology, University of South Florida, College of Medicine, Tampa, Florida, USA aut NATIONALLICENCE 700 1- Gonzalvo Americo Department of Pathology, University of South Florida, College of Medicine, Tampa, Florida, USA aut NATIONALLICENCE 700 1- Saporta Samuel Department of Anatomy, University of South Florida, College of Medicine, Tampa, Florida, USA aut NATIONALLICENCE 700 1- Gonzalez Orlando Department of Pathology, University of South Florida, College of Medicine, Tampa, Florida, USA aut NATIONALLICENCE 700 1- Prockop Leon Department of Neurology, University of South Florida, College of Medicine, Tampa, Florida, USA aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 14/3(1992-11-01), 225-236 0167-594X 14:3<225 1992 14 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer