caa a22 4500 469117125 CHVBK 20180323133128.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00177613 doi (NATIONALLICENCE)springer-10.1007/BF00177613 Growth of human acoustic neuromas, neurofibromas and schwannomas in the subrenal capsule and sciatic nerve of the nude mouse [Elektronische Daten] [Jung Lee, Raymond Sobel, E. Chiocca, Tai Kim, Robert Martuza] To develop a reproducible in vivo model for the growth of human acoustic neuromas and neurofibromas, we implanted tumor specimens (6 acoustic neuromas; 4 neurofibromas; 3 schwannomas arising in skin and soft tissues) from 13 different patients into the subrenal capsules of 67 nude mice and sciatic nerves of 64 nude mide. The animals were anesthetized and the tumors were microscopically implanted. Serial tumor volumes were determined at intervals up to 2 months by reopening the incision and directly measuring the tumor size with a micrometer. The percentages of acoustic neuromas that survived or grew were 57.1% in the subrenal capsule and 88.9% in the sciatic nerves; the percentages of neurofibromas that survived and grew were 50% in the subrenal capsule and 70% in the sciatic nerves; and the percentages of schwannomas that survived and grew were 57.1% in the subrenal capsule and 94.1% in the sciatic nerve. Tumors in the sciatic nerve also survived and grew for a longer period than those in the subrenal capsules. Tumor enlargement and stability correlated with neovascularity. At 1 or 2 months after engraftment, the tumors showed histologic appearances similar to the original tumors and immunohistochemical analysis of cryostat sections demonstrated staining of the tumors, but not the host mouse tissues for human β2-microglobulin, a species-specific marker. Furthermore, analysis of genomic DNA from implanted tumors revealed its human origin. We conclude that human acoustic neuromas, neurofibromas and schwannomas are readily grown in two sites in nude mice and that they retain their morphologic features and genomic identities. These tumors grow better and more consistently in the sciatic nerve than in the subrenal capsule. These are useful model systems for studying tumor growth and cellular modulation. Kluwer Academic Publishers, 1992 acoustic neuroma nationallicence neurofibroma nationallicence nude mouse nationallicence schwannoma nationallicence sciatic nerve nationallicence subrenal capsule nationallicence xenograft nationallicence Lee Jung Department of Surgery (Neurosurgery Service), Massachusetts General Hospital and Harvard Medical School, Boston, Mass., USA aut Sobel Raymond Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Mass., USA aut Chiocca E. Department of Surgery (Neurosurgery Service), Massachusetts General Hospital and Harvard Medical School, Boston, Mass., USA aut Kim Tai Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Mass., USA aut Martuza Robert Department of Surgery (Neurosurgery Service), Massachusetts General Hospital and Harvard Medical School, Boston, Mass., USA aut Journal of Neuro-Oncology Kluwer Academic Publishers 14/2(1992-10-01), 101-112 0167-594X 14:2<101 1992 14 11060 https://doi.org/10.1007/BF00177613 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00177613 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Jung Department of Surgery (Neurosurgery Service), Massachusetts General Hospital and Harvard Medical School, Boston, Mass., USA aut NATIONALLICENCE 700 1- Sobel Raymond Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Mass., USA aut NATIONALLICENCE 700 1- Chiocca E. Department of Surgery (Neurosurgery Service), Massachusetts General Hospital and Harvard Medical School, Boston, Mass., USA aut NATIONALLICENCE 700 1- Kim Tai Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Mass., USA aut NATIONALLICENCE 700 1- Martuza Robert Department of Surgery (Neurosurgery Service), Massachusetts General Hospital and Harvard Medical School, Boston, Mass., USA aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 14/2(1992-10-01), 101-112 0167-594X 14:2<101 1992 14 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer