caa a22 4500 469118571 CHVBK 20180323133131.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF02245879 doi (NATIONALLICENCE)springer-10.1007/BF02245879 Anxiolytic effects of nitrous oxide in mice in the light-dark and holeboard exploratory tests [Elektronische Daten] [Donald Czech, Dena Green] To investigate anxiolytic effects of nitrous oxide (N2O), male mice were tested in two exploratory models — a two-chambered light-dark (L-D) unit and a holeboard. Tests were conducted inside a glovebag through which one of three mixtures of N2O and oxygen (25, 50 and 75% N2O) or room air (RA) was circulated at a flow rate of 101/min. The principal findings in the L-D unit were a concentration-related increase in number of interdepartmental transitions and a generalized increase in time spent on the light side. Nitrous oxide effectively elevated transitions in the L-D unit at a lower concentration (25% N2O) than was required to increase locomotor activity in an open field (50% N2O), suggesting that these two measures are at least partially independent; transitions might reflect a specific exploratory component of locomotor behavior. In the holeboard test, a concentration-related increase in number of head dips was observed. Pretreatment with naltrexone-HCl or saline vehicle revealed a contribution by an endogenous opioid-linked locomotor stimulant effect in some measures. A dose-related reversal by flumazenil of 50% N2O-induced shifts in number of head dips and time spent head-dipping implicates a benzodiazepine receptor. Both paradigms, in particular the holeboard, should prove useful in future N2O research. Springer-Verlag, 1992 Anxiolytic nationallicence Nitrous oxide nationallicence Light-dark exploratory test nationallicence Holeboard test nationallicence Exploratory nationallicence Locomotor nationallicence Flumazenil nationallicence Mice nationallicence Czech Donald Department of Psychology (SC-454), Marquette University, 53233, Milwaukee, WI, USA aut Green Dena Department of Psychology (SC-454), Marquette University, 53233, Milwaukee, WI, USA aut Psychopharmacology Springer-Verlag 109/3(1992-11-01), 315-320 0033-3158 109:3<315 1992 109 213 https://doi.org/10.1007/BF02245879 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02245879 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Czech Donald Department of Psychology (SC-454), Marquette University, 53233, Milwaukee, WI, USA aut NATIONALLICENCE 700 1- Green Dena Department of Psychology (SC-454), Marquette University, 53233, Milwaukee, WI, USA aut NATIONALLICENCE 773 0- Psychopharmacology Springer-Verlag 109/3(1992-11-01), 315-320 0033-3158 109:3<315 1992 109 213 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer