caa a22 4500 469119071 CHVBK 20180323133133.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF02245478 doi (NATIONALLICENCE)springer-10.1007/BF02245478 Parametric and pharmacological analyses of the enhanced grooming response elicited by the D1 dopamine receptor agonist SKF 38393 in the rat [Elektronische Daten] [Stephen Wachtel, Richard Brooderson, Francis White] The present report investigated several parametric and pharmacological aspects of the enhanced self-grooming behavior of rats following systemic administration of the selective D1 dopamine (DA) receptor agonist SKF 38393. The amount of time that rats spent grooming themselves was measured continuously for 30 min following drug administration to provide a quantitative measure of the drug-induced behavior. SKF 38393 increased the amount of grooming in a dose-dependent manner (0.5-16 mg/kg, SC). The onset of this effect required at least 5 min and it persisted for at least 60 min. The ability of SKF 38393 to enhance grooming was shared by R-SKF 38393, but not S-SKF 38393, consistent with the affinities of these enantiomers for the D1 DA receptor. Unlike SKF 38393, the peripheral D1 agonist fenoldopam (SKF 82526) failed to cause an increased grooming response, suggesting a central site of action for elicitation of this behavior. The SKF 38393-induced increase in grooming was competitively antagonized by the D1 selective antagonist SCH 23390 (0.5 mg/kg, SC). Although the D2 DA receptor-selective antagonist eticlopride reduced SKF 38393-elicited grooming, this antagonism appeared to be of a physiological rather than pharmacological nature. When eticlopride was coadministered with the non-selective (mixed) D1/D2 agonist apomorphine, an increase in grooming behavior similar to that produced by SKF 38393 was observed. Inactivation of D1 and D2 DA receptors produced by pretreatment with the irreversible antagonistN-ethoxycarbonyl-2-ethoxy-1, 2-dihydroquinoline (EEDQ), at a dose which reduces D1 and D2 receptor density by ≥50% (8.0 mg/kg, IP), reduced SKF 38393-induced grooming by approximately 50%. Prior protection of D1 receptors by SCH 23390 completely prevented the effect of EEDQ whereas prior protection of D2 receptors by eticlopride did not. These results demonstrate that enhanced grooming behavior elicited by dopamine agonists in rats, when measured as the amount of time spent grooming, provides a reliable, quantifiable index of selective D1 DA receptor activation in the CNS. In addition, this behavior does not appear to require concurrent stimulation of D2 DA receptors by endogenous DA. Springer-Verlag, 1992 D1 receptors nationallicence D2 receptors nationallicence Grooming nationallicence SKF 38393 nationallicence Fenoldopam nationallicence SCH 23390 nationallicence Synergism nationallicence Wachtel Stephen Departments of Psychiatry and Pharmacology, Wayne State University School of Medicine, Neuropsychopharmacology Laboratory, Lafayette Clinic, 951 East Lafayette, 48207, Detroit, MI, USA aut Brooderson Richard Departments of Psychiatry and Pharmacology, Wayne State University School of Medicine, Neuropsychopharmacology Laboratory, Lafayette Clinic, 951 East Lafayette, 48207, Detroit, MI, USA aut White Francis Departments of Psychiatry and Pharmacology, Wayne State University School of Medicine, Neuropsychopharmacology Laboratory, Lafayette Clinic, 951 East Lafayette, 48207, Detroit, MI, USA aut Psychopharmacology Springer-Verlag 109/1-2(1992-10-01), 41-48 0033-3158 109:1-2<41 1992 109 213 https://doi.org/10.1007/BF02245478 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02245478 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wachtel Stephen Departments of Psychiatry and Pharmacology, Wayne State University School of Medicine, Neuropsychopharmacology Laboratory, Lafayette Clinic, 951 East Lafayette, 48207, Detroit, MI, USA aut NATIONALLICENCE 700 1- Brooderson Richard Departments of Psychiatry and Pharmacology, Wayne State University School of Medicine, Neuropsychopharmacology Laboratory, Lafayette Clinic, 951 East Lafayette, 48207, Detroit, MI, USA aut NATIONALLICENCE 700 1- White Francis Departments of Psychiatry and Pharmacology, Wayne State University School of Medicine, Neuropsychopharmacology Laboratory, Lafayette Clinic, 951 East Lafayette, 48207, Detroit, MI, USA aut NATIONALLICENCE 773 0- Psychopharmacology Springer-Verlag 109/1-2(1992-10-01), 41-48 0033-3158 109:1-2<41 1992 109 213 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer