caa a22 4500 469119357 CHVBK 20180323133134.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF02245124 doi (NATIONALLICENCE)springer-10.1007/BF02245124 Effects of acute and chronic desipramine treatment on somatostatin receptors in brain [Elektronische Daten] [Evanghelia Gheorvassaki, Kyriaki Thermos, George Liapakis, Christina Spyraki] The effects of acute (5 mg/kg, IP twice daily for 2 days) and chronic (5 mg/kg IP twice daily for 21 days) administration of desipramine (DMI) on [125I]-Tyr11-somatostatin binding sites in brain were examined. There was no change in [125I]Tyr11-somatostatin binding in membranes prepared from the frontal cortex, striatum, and hippocampus of rats acutely or chronically treated with DMI as compared to non treated animals. [125I]Tyr11-Somatostatin binding was increased in membranes prepared from the rat nucleus accumbens only after chronic DMI administration. Scatchard analysis of the binding data from the nucleus accumbens showed that [125I]Tyr11-somatostatin labels a single population of somatostatin binding sites with an affinity constant, Kd, of 1.8±0.60 nM and a Bmax of 330±90 fmol/mg protein. Chronic treatment with DMI increased the Bmax (500±140 fmol/mg protein) but had no effect on the Kd. This finding shows a regional effect of DMI on [125I]Tyr11-somatostatin binding sites in rat brain and suggests that somatostatin may play a role in the pathophysiology of depression. Springer-Verlag, 1992 Desipramine nationallicence [125I]Tyr11-somatostatin binding sites nationallicence Rat brain nationallicence Gheorvassaki Evanghelia Laboratory of Pharmacology, Department of Basic Sciences, Division of Medicine, School of Health Sciences, University of Crete, 71409, Heraklion, Greece aut Thermos Kyriaki Laboratory of Pharmacology, Department of Basic Sciences, Division of Medicine, School of Health Sciences, University of Crete, 71409, Heraklion, Greece aut Liapakis George Laboratory of Pharmacology, Department of Basic Sciences, Division of Medicine, School of Health Sciences, University of Crete, 71409, Heraklion, Greece aut Spyraki Christina Laboratory of Pharmacology, Department of Basic Sciences, Division of Medicine, School of Health Sciences, University of Crete, 71409, Heraklion, Greece aut Psychopharmacology Springer-Verlag 108/3(1992-08-01), 363-366 0033-3158 108:3<363 1992 108 213 https://doi.org/10.1007/BF02245124 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02245124 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gheorvassaki Evanghelia Laboratory of Pharmacology, Department of Basic Sciences, Division of Medicine, School of Health Sciences, University of Crete, 71409, Heraklion, Greece aut NATIONALLICENCE 700 1- Thermos Kyriaki Laboratory of Pharmacology, Department of Basic Sciences, Division of Medicine, School of Health Sciences, University of Crete, 71409, Heraklion, Greece aut NATIONALLICENCE 700 1- Liapakis George Laboratory of Pharmacology, Department of Basic Sciences, Division of Medicine, School of Health Sciences, University of Crete, 71409, Heraklion, Greece aut NATIONALLICENCE 700 1- Spyraki Christina Laboratory of Pharmacology, Department of Basic Sciences, Division of Medicine, School of Health Sciences, University of Crete, 71409, Heraklion, Greece aut NATIONALLICENCE 773 0- Psychopharmacology Springer-Verlag 108/3(1992-08-01), 363-366 0033-3158 108:3<363 1992 108 213 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer