caa a22 4500 469120304 CHVBK 20180323133136.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF02244977 doi (NATIONALLICENCE)springer-10.1007/BF02244977 Acute ethanol intoxication may not alter α2-adrenoceptors in the human brain [Elektronische Daten] [J. Javier Meana, Ane Gabilondo, Fernando Barturen, Jesús García-Sevilla] The specific binding of the α2-adrenoceptor agonists [3H]clonidine and [3H]bromoxidine (UK 14304) was measured in the postmortem brain of ethanol intoxicated nonalcoholic subjects (blood ethanol concentration: 1.37±0.30 g/l) and matched controls. In the frontal cortex, the density of binding sites for [3H]clonidine (Bmax=58±7 fmol/mg protein) and [3H]bromoxidine (UK 14304) (Bmax=49±7 fmol/mg protein) in ethanol intoxicated subjects did not differ from those in the control groups (Bmax=68±4 and 56±8 fmol/mg protein for the respective radioligand). The dissociation constants (KD) were also similar in both groups. The binding capacities (Bmax) and KD values for both radioligands also were found unchanged in the hypothalamus, amygdala, head of caudate, hippocampus and cerebellum. The results demonstrate that, contrary to the β-adrenoceptor, the presence of ethanol in the human brain does not alter the high-affinity state of the α2-adrenoceptor in the frontal cortex and possibly also in other brain regions. Springer-Verlag, 1992 Ethanol nationallicence Human brain nationallicence α2-Adrenoceptors nationallicence [3H]Clonidine nationallicence [3H]Bromoxidine (UK 14304) nationallicence Javier Meana J. Section of Pharmacology, Department of Neurosciences, University of the Basque Country, Leioa, Bizkaia aut Gabilondo Ane Section of Pharmacology, Department of Neurosciences, University of the Basque Country, Leioa, Bizkaia aut Barturen Fernando Section of Pharmacology, Department of Neurosciences, University of the Basque Country, Leioa, Bizkaia aut García-Sevilla Jesús Section of Pharmacology, Department of Neurosciences, University of the Basque Country, Leioa, Bizkaia aut Psychopharmacology Springer-Verlag 107/1(1992-05-01), 132-134 0033-3158 107:1<132 1992 107 213 https://doi.org/10.1007/BF02244977 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02244977 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Javier Meana J. Section of Pharmacology, Department of Neurosciences, University of the Basque Country, Leioa, Bizkaia aut NATIONALLICENCE 700 1- Gabilondo Ane Section of Pharmacology, Department of Neurosciences, University of the Basque Country, Leioa, Bizkaia aut NATIONALLICENCE 700 1- Barturen Fernando Section of Pharmacology, Department of Neurosciences, University of the Basque Country, Leioa, Bizkaia aut NATIONALLICENCE 700 1- García-Sevilla Jesús Section of Pharmacology, Department of Neurosciences, University of the Basque Country, Leioa, Bizkaia aut NATIONALLICENCE 773 0- Psychopharmacology Springer-Verlag 107/1(1992-05-01), 132-134 0033-3158 107:1<132 1992 107 213 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer