caa a22 4500 469121629 CHVBK 20180323133140.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF02246226 doi (NATIONALLICENCE)springer-10.1007/BF02246226 Mode of action and characteristics of monoamine oxidase-A inhibition by moclobemide [Elektronische Daten] [A. Cesura, R. Kettler, R. Imhof, M. Da Prada] The mode of interaction of the reversible monoamine oxidase-A (MAO-A) inhibitor moclobemide with the enzyme was investigated. The inhibition of rat brain or human placenta MAO-A by moclobemide showed an initial competitive phase, with a relatively low affinity (KI=0.2-0.4 mM). However, the potency of the inhibitor was increased with incubation time. The time-dependent component of the association of moclobemide with MAO-A followed pseudo-first order kinetics. In contrast to mechanism-based inhibitors, no indication for adduct or product formation was detected after incubation of moclobemide with the enzyme. Even though some aspects of the moclobemide interaction with MAO-A are still not completely elucidated, this compound seems to have the characteristics of a slow-binding inhibitor. Springer-Verlag, 1992 Moclobemine nationallicence Monoamine oxidase-A nationallicence Inhibition nationallicence Cesura A. Pharmaceutical Research Department, F. Hoffmann-La Roche Ltd, CH-4002, Basel, Switzerland aut Kettler R. Pharmaceutical Research Department, F. Hoffmann-La Roche Ltd, CH-4002, Basel, Switzerland aut Imhof R. Pharmaceutical Research Department, F. Hoffmann-La Roche Ltd, CH-4002, Basel, Switzerland aut Da Prada M. Pharmaceutical Research Department, F. Hoffmann-La Roche Ltd, CH-4002, Basel, Switzerland aut Psychopharmacology Springer-Verlag 106(1992-02-01), S15-S16 0033-3158 106<S15 1992 106 213 https://doi.org/10.1007/BF02246226 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02246226 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cesura A. Pharmaceutical Research Department, F. Hoffmann-La Roche Ltd, CH-4002, Basel, Switzerland aut NATIONALLICENCE 700 1- Kettler R. Pharmaceutical Research Department, F. Hoffmann-La Roche Ltd, CH-4002, Basel, Switzerland aut NATIONALLICENCE 700 1- Imhof R. Pharmaceutical Research Department, F. Hoffmann-La Roche Ltd, CH-4002, Basel, Switzerland aut NATIONALLICENCE 700 1- Da Prada M. Pharmaceutical Research Department, F. Hoffmann-La Roche Ltd, CH-4002, Basel, Switzerland aut NATIONALLICENCE 773 0- Psychopharmacology Springer-Verlag 106(1992-02-01), S15-S16 0033-3158 106<S15 1992 106 213 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer