caa a22 4500 469126043 CHVBK 20180323133151.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF01623073 doi (NATIONALLICENCE)springer-10.1007/BF01623073 Quantitation of carbohydrate sweeteners and organic acids in human oral fluid using HPLC analysis [Elektronische Daten] [H. Linke, S. Moss] Bestimmung von Zuckern und organischen Säuren in menschlicher Mundflüssigkeit unter Verwendung der HPLC-Analyse Summary: A sensitive high performance liquid chromatography (HPLC) assay was developed for the qualitative and quantitative determination of carbohydrate sweeteners and organic acids in oral fluid. To separate these compounds, an ion-moderated partition resin HPLC column (Aminex HPX-87H) was used. All components of the HPLC system were interconnected using stainless steel capillary tubing. Isocratic elution with 0.01 N sulfuric acid provided the profile of both compound classes. The compounds were detected using a refractive index detector. The method employed computerized data collection and integration (Omega-2 system) with a detection sensitivity of 0.1 µg compound per HPLC assay (80 µl). This method is useful in caries research, because it detects minute amounts of sugars and organic acids in oral fluid during clearance studies of various foods in the mouth. Steinkopff-Verlag, 1992 S ugars nationallicence c arbohydrates nationallicence l actic a cid nationallicence o ral f luid nationallicence H PLC nationallicence g lucose nationallicence s ucrose nationallicence Zucker nationallicence Kohlenhydrate nationallicence Milchsäure nationallicence Speichel nationallicence HPLC-Analyse nationallicence Glukose nationallicence Saccharose nationallicence Linke H. Departments of Microbiology and Pediatric Dentistry, New York University Dental Center, USA aut Moss S. Departments of Microbiology and Pediatric Dentistry, New York University Dental Center, USA aut Zeitschrift für Ernährungswissenschaft Steinkopff-Verlag 31/2(1992-06-01), 147-154 0044-264X 31:2<147 1992 31 394 https://doi.org/10.1007/BF01623073 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01623073 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Linke H. Departments of Microbiology and Pediatric Dentistry, New York University Dental Center, USA aut NATIONALLICENCE 700 1- Moss S. Departments of Microbiology and Pediatric Dentistry, New York University Dental Center, USA aut NATIONALLICENCE 773 0- Zeitschrift für Ernährungswissenschaft Steinkopff-Verlag 31/2(1992-06-01), 147-154 0044-264X 31:2<147 1992 31 394 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer