caa a22 4500 46912640X CHVBK 20180323133152.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF02705129 doi (NATIONALLICENCE)springer-10.1007/BF02705129 Choi Jeong-Woo Department of Chemical Engineering, Sogang University, C.P.O. Box 1142, Seoul, Korea aut In situ berberine separation with immobilized adsorbent in cell suspension cultures of Thalictrum rugosum [Elektronische Daten] [Jeong-Woo Choi] The use of suspended and alginate-entrapped XAD-7, polycarboxyl ester resin, for thein situ separation of berberine, isoquinoline alkaloid, produced from plant cell culture ofThalictrum rugosum was investigated. XAD-7 could adsorb the berberine and the amount of berberine adsorbed on XAD-7 depended on pH. The neutral form of berberine was adsorbed onto XAD-7 and the adsorption isotherm for berberine showed a Langmuir-type appearance.In situ berberine removal enhanced the production of secondary metabolites in cell suspension culture ofThalictrum rugosum. Addition of XAD-7 at the exponential phase of cell growth was the most effective for enhancement of berberine production. In chitosan-treated cell culture to permeabilize intracellular berberine, berberine secretion was significantly accelerated by addition of alginate-entrapped XAD-7 at the stationary phase of cell growth and thus more than 70% of the produced berberine could be adsorbed to alginate-entrapped XAD-7. Korean Institute of Chemical Engineering, 1992 Korean Journal of Chemical Engineering Springer-Verlag 9/3(1992-07-01), 128-134 0256-1115 9:3<128 1992 9 11814 https://doi.org/10.1007/BF02705129 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02705129 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Choi Jeong-Woo Department of Chemical Engineering, Sogang University, C.P.O. Box 1142, Seoul, Korea aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer-Verlag 9/3(1992-07-01), 128-134 0256-1115 9:3<128 1992 9 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer