caa a22 4500 469126477 CHVBK 20180323133152.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF02705296 doi (NATIONALLICENCE)springer-10.1007/BF02705296 Experimental studies on phase equilibria of PEG-water-dextran/ammonium sulfate systems and partitioning of albumin into two water-continuous phases [Elektronische Daten] [Bong Kim, Yong-Byung Ban, Jong-Duk Kim] The clouding points and equilibrium concentrations of PEG/dextran/water and PEG /ammonium sulfate/water systems were experimentally determined for different molecular weights of PEG by titration method and direct determination of concentrations. In phase diagrams of PEG-water with ammonium sulfate or dextran, the addition of salt or dextran induced the phase separation of PEG-rich phases near the PEG-lean phases having the different partitioning of PEG. The concentrations of PEG in PEG-rich phases increase as the amounts of PEG or salt and dextran increase, while the concentrations of PEG in aqueous media decrease in any cases. The higher the molecular weight of PEG has, the wider the two-phase regions are. In dextran DT10 systems, the partition coefficients of egg albumin into PEG-lean phases increase with concentrations of dextran and the molecular weight of PEG. In ammonium sulfate systems, the partitioning coefficients showed a maximum, having lower partitioning at the very high and low concentration of salts. It is also observed that as the amounts of albumin increase, the partitioning of albumin into PEG-lean phase increases. Korean Institute of Chemical Engineering, 1992 Kim Bong Department of Chemical Engineering and Bioprocess ERC, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-ku, 305-701, Taejon aut Ban Yong-Byung Department of Chemical Engineering and Bioprocess ERC, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-ku, 305-701, Taejon aut Kim Jong-Duk Department of Chemical Engineering and Bioprocess ERC, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-ku, 305-701, Taejon aut Korean Journal of Chemical Engineering Springer-Verlag 9/4(1992-10-01), 219-224 0256-1115 9:4<219 1992 9 11814 https://doi.org/10.1007/BF02705296 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02705296 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Bong Department of Chemical Engineering and Bioprocess ERC, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-ku, 305-701, Taejon aut NATIONALLICENCE 700 1- Ban Yong-Byung Department of Chemical Engineering and Bioprocess ERC, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-ku, 305-701, Taejon aut NATIONALLICENCE 700 1- Kim Jong-Duk Department of Chemical Engineering and Bioprocess ERC, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-ku, 305-701, Taejon aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer-Verlag 9/4(1992-10-01), 219-224 0256-1115 9:4<219 1992 9 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer