caa a22 4500 469126523 CHVBK 20180323133152.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF02697532 doi (NATIONALLICENCE)springer-10.1007/BF02697532 Effect of sample sizes on peak shapes in preparative liquid chromatography [Elektronische Daten] [Kyung Row, Youn Lee] Cyclobutane pyrimidine dimers and monomers of thymine were separated on a C18 reversed-phase high performance liquid Chromatographic column. Using the two mathematical models, the effect of the sample sizes on peak shapes in preparative liquid chromatography was investigated. One of them is the linear kinetic model, and the other is based on the nonlinear adsorption isotherm. In case of small injections of sample, the good agreements between the calculated value by the linear kinetic model and experimental data were achieved. However, when sample size is increased, this model lacks in the prediction of large concentration profile of sample. However, the nonlinear model permits the accurate prediction of the location of the component ▪ band and the determination of the appropriate time to start and stop collection of the enriched fraction at the higher concentrations of monomer and the lower that of dimer. Therefore, extremely small amounts of dimer can be extracted from monomer solutions. Korean Institute of Chemical Engineering, 1992 Row Kyung CFC Alternatives Technology Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, Korea aut Lee Youn CFC Alternatives Technology Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, Korea aut Korean Journal of Chemical Engineering Springer-Verlag 9/2(1992-04-01), 83-88 0256-1115 9:2<83 1992 9 11814 https://doi.org/10.1007/BF02697532 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02697532 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Row Kyung CFC Alternatives Technology Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Youn CFC Alternatives Technology Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, Korea aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer-Verlag 9/2(1992-04-01), 83-88 0256-1115 9:2<83 1992 9 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer