caa a22 4500 469127279 CHVBK 20180323133153.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF02357226 doi (NATIONALLICENCE)springer-10.1007/BF02357226 Anaplasmosis in Uganda. I. Use of dried blood on filter papers and serum samples for serodiagnosis of anaplasmosis—A comparative study [Elektronische Daten] [G. Ssenyonga, I. Kakoma, Sonia Montenegro-James, R. Hansen] Summary: The suitability of blood collected on filter papers in comparison with corresponding conventional serum samples in the diagnosis of bovine anaplasmosis was studied using the complement fixation test, DOT-ELISA, Western immunoblot and rapid card agglutination test. Dried blood on Whatman filter paper no. 1 was eluted in PBS 0·05% Tween 20 giving an initial dilution of 1∶10. The reactivity of the eluted samples in both DOT-ELISA and Western immunoblotting were similar to those obtained with the corresponding straight serum sample dilutions. Filter paper samples gave lower reactivity in the remaining tests when compared with corresponding serum samples. There was no significant difference in the reactivity between the eluates from filter papers stored at temperatures ranging between 15·5 and 24°C and those kept refrigerated. Storage at 15·5 to 24°C did not significantly affect reactivity for up to six months. Eluates from filter papers stored for six months at 15·5 to 24°C continued to give similar reactivity as those from freshly prepared filter papers in both DOT-ELISA and Western blot, and in the rapid card agglutination test. It is concluded that collecting blood on filter papers is a suitable technique for large scale seroepidemiological studies on anaplasmosis and offers many advantages in developing countries where transport and cold chain facilities are a major constraint. Centre for Tropical Veterinary Medicine, University of Edinburgh, 1992 Ssenyonga G. Faculty of Veterinary Medicine, Makerere University, PO Box 7062, Kampala, Uganda aut Kakoma I. College of Veterinary Medicine, University of Illinois, 61801, Urbana, Illinois, USA aut Montenegro-James Sonia College of Veterinary Medicine, University of Illinois, 61801, Urbana, Illinois, USA aut Hansen R. College of Veterinary Medicine, University of Illinois, 61801, Urbana, Illinois, USA aut Tropical Animal Health and Production Kluwer Academic Publishers 24/1(1992-03-01), 2-8 0049-4747 24:1<2 1992 24 11250 https://doi.org/10.1007/BF02357226 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02357226 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ssenyonga G. Faculty of Veterinary Medicine, Makerere University, PO Box 7062, Kampala, Uganda aut NATIONALLICENCE 700 1- Kakoma I. College of Veterinary Medicine, University of Illinois, 61801, Urbana, Illinois, USA aut NATIONALLICENCE 700 1- Montenegro-James Sonia College of Veterinary Medicine, University of Illinois, 61801, Urbana, Illinois, USA aut NATIONALLICENCE 700 1- Hansen R. College of Veterinary Medicine, University of Illinois, 61801, Urbana, Illinois, USA aut NATIONALLICENCE 773 0- Tropical Animal Health and Production Kluwer Academic Publishers 24/1(1992-03-01), 2-8 0049-4747 24:1<2 1992 24 11250 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer