caa a22 4500 469128062 CHVBK 20180323133156.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF01809858 doi (NATIONALLICENCE)springer-10.1007/BF01809858 Possible prebiotic significance of polyamines in the condensation, protection, encapsulation, and biological properties of DNA [Elektronische Daten] [Isabel Baeza, Miguel Ibáñez, Carlos Wong, Pedro Chávez, Patricio Gariglio, J. Oró] Some properties of DNA condensed with spermidine have been compared with the properties of DNA condensed with Co3+(NH3)6 to determine whether condensation of DNA with these trivalent cations protects DNA against the action of DNase I and increases transcription and encapsulation of DNA into liposomes. It was shown that DNA condensed with Co3+(NH3)6 was resistant to the action of the endonuclease DNase I such as DNA condensed with spermidine was. However, DNA condensed with Co3+(NH3)6 was significantly less active in transcription with theE. coli RNA polymerase than DNA-spermidine condensed forms. In addition, it was demonstrated that both compacted forms of DNA were more efficiently encapsulated into neutral liposomes; however, negatively, charged liposomes were scarcely formed in the presence of DNA condensed with Co3+(NH3)6. These experiments and the well documented properties of polyamines increasing the resistance to radiations and hydrolysis of nucleic acids, as well as their biological activities, such as replication, transcription, and translation, together with the low concentration of Co3+ in the environment, lead us to propose spermidine as a plausible prebiotic DNA condensing agent rather than Co3+ and the basic proteins proposed by other authors. Then, we consider the possible role and relevance of the polyamine-nucleic acids complexes in the evolution of life. Kluwer Academic Publishers, 1992 Baeza Isabel Departamento de Bioquímica, Escuela Nacional de Ciencias Biológicas, IPN 11340, México, D.E. México aut Ibáñez Miguel Departamento de Bioquímica, Escuela Nacional de Ciencias Biológicas, IPN 11340, México, D.E. México aut Wong Carlos Departamento de Bioquímica, Escuela Nacional de Ciencias Biológicas, IPN 11340, México, D.E. México aut Chávez Pedro Departamento de Genética y Biología Molecular, Centro de Investigación y Estudios Avanzados, IPN 07000, México, D.F. México aut Gariglio Patricio Departamento de Genética y Biología Molecular, Centro de Investigación y Estudios Avanzados, IPN 07000, México, D.F. México aut Oró J. Department of Biochemical and Biophysical Sciences, University of Houston, 77204, Houston, TX, USA aut Origins of life and evolution of the biosphere Kluwer Academic Publishers 21/4(1992-07-01), 225-242 0169-6149 21:4<225 1992 21 11084 https://doi.org/10.1007/BF01809858 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01809858 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baeza Isabel Departamento de Bioquímica, Escuela Nacional de Ciencias Biológicas, IPN 11340, México, D.E. México aut NATIONALLICENCE 700 1- Ibáñez Miguel Departamento de Bioquímica, Escuela Nacional de Ciencias Biológicas, IPN 11340, México, D.E. México aut NATIONALLICENCE 700 1- Wong Carlos Departamento de Bioquímica, Escuela Nacional de Ciencias Biológicas, IPN 11340, México, D.E. México aut NATIONALLICENCE 700 1- Chávez Pedro Departamento de Genética y Biología Molecular, Centro de Investigación y Estudios Avanzados, IPN 07000, México, D.F. México aut NATIONALLICENCE 700 1- Gariglio Patricio Departamento de Genética y Biología Molecular, Centro de Investigación y Estudios Avanzados, IPN 07000, México, D.F. México aut NATIONALLICENCE 700 1- Oró J. Department of Biochemical and Biophysical Sciences, University of Houston, 77204, Houston, TX, USA aut NATIONALLICENCE 773 0- Origins of life and evolution of the biosphere Kluwer Academic Publishers 21/4(1992-07-01), 225-242 0169-6149 21:4<225 1992 21 11084 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer