caa a22 4500 469128119 CHVBK 20180323133156.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF01809372 doi (NATIONALLICENCE)springer-10.1007/BF01809372 Mixed anhydrides (phosphoric-carboxyl) are also formed in the esterification of 5′-amp with n-acetylaminoacyl imidazolides: Implications regarding the origin of protein synthesis [Elektronische Daten] [Nalinie Wickramasinghe, James Lacey Jr.] Procedures for the formation of aminoacyl esters of monoribonucleotides with aminoacyl imidazolides were first reported by Gottikhet al. and summarized in 1970. This reaction has been widely used by us and numbers of other workers as a convenient means of preparing aminoacyl esters of nucleotides. We have previously reported that, under conditions of excess imidazolide, large amounts of bis 2′, 3′ esters are formed in addition to the monoesters, (Laceyet al., 1991). However, to our knowledge, no one has reported that in addition to the esters, relatively large amounts of the mixed anhydride, with the amino acid carboxyl attached to the phosphate, are also formed at short reaction times. We report here on the relative amounts of anhydride and esters formed in this reaction of racemic mixtures of eleven N-acetyl amino acid imidazolides with 5′-AMP and discuss the relevance of the findings to the origin of protein synthesis. Kluwer Academic Publishers, 1992 Wickramasinghe Nalinie Department of Biochemistry, University of Alabama at Birmingham, 35294, Birmingham, AL, USA aut Lacey Jr. James Department of Biochemistry, University of Alabama at Birmingham, 35294, Birmingham, AL, USA aut Origins of life and evolution of the biosphere Kluwer Academic Publishers 22/6(1992-11-01), 361-368 0169-6149 22:6<361 1992 22 11084 https://doi.org/10.1007/BF01809372 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01809372 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wickramasinghe Nalinie Department of Biochemistry, University of Alabama at Birmingham, 35294, Birmingham, AL, USA aut NATIONALLICENCE 700 1- Lacey Jr James Department of Biochemistry, University of Alabama at Birmingham, 35294, Birmingham, AL, USA aut NATIONALLICENCE 773 0- Origins of life and evolution of the biosphere Kluwer Academic Publishers 22/6(1992-11-01), 361-368 0169-6149 22:6<361 1992 22 11084 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer