caa a22 4500 469128127 CHVBK 20180323133156.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF01809373 doi (NATIONALLICENCE)springer-10.1007/BF01809373 Oligomerization reactions of ribonucleotides: The reaction of the 5′-phosphorimidazolide of nucleosides on montmorillonite and other minerals [Elektronische Daten] [James Ferris, Gözen Ertem] The reaction of ImpA in the presence of Na+-montmorillonite 22A or Na+-Volclay in aqueous, pH 8 solution gives a 50-60% yield of dimers and trimers (pA)2 and (pA)3. The ratio of 3′,5′-phosphodiester bond formation is twice as great as 2′,5′-bond formation. The reaction requires the presence of Mg2+ and is inhibited by 0.4 M imidazole. N-methylimidazole enhances the rate of the reaction but does not cause major changes in yield or product composition. Higher yields were obtained when Li+- or Ca2+-montmorillonites were used in place of Na+-montmorillonite. Little or no phosphodiester bond formation was observed with Mg2+- or Al3+-montmorillonite. Montmorillonites other than 22A and Volclay exhibited little or no catalysis. In addition, little or no catalysis was exhibited in ferrugenous smectite, nontronite, allophane, imogolite or sepiolite. Oligomers were also formed by the reaction of ImpG, 2-methylImpG, ImpC and ImpU in the presence of Na+-montmorillonite. The pyrimidine nucleotides gave significantly lower yields of oligomers. Kluwer Academic Publishers, 1992 Ferris James Department of Chemistry, Rensselaer Polytechnic Institute, 12180-3590, Troy, NY, USA aut Ertem Gözen Department of Chemistry, Rensselaer Polytechnic Institute, 12180-3590, Troy, NY, USA aut Origins of life and evolution of the biosphere Kluwer Academic Publishers 22/6(1992-11-01), 369-381 0169-6149 22:6<369 1992 22 11084 https://doi.org/10.1007/BF01809373 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01809373 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ferris James Department of Chemistry, Rensselaer Polytechnic Institute, 12180-3590, Troy, NY, USA aut NATIONALLICENCE 700 1- Ertem Gözen Department of Chemistry, Rensselaer Polytechnic Institute, 12180-3590, Troy, NY, USA aut NATIONALLICENCE 773 0- Origins of life and evolution of the biosphere Kluwer Academic Publishers 22/6(1992-11-01), 369-381 0169-6149 22:6<369 1992 22 11084 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 469128127 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer