caa a22 4500 469128437 CHVBK 20180323133157.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00573496 doi (NATIONALLICENCE)springer-10.1007/BF00573496 Na+/H+ antiporter properties in peripheral blood lymphocytes from normotensive obese and type 2 diabetic patients do not differ significantly from controls [Elektronische Daten] [D. Ghigo, P. Alessio, S. Burzacca, C. Costamagna, G. Anfossi, F. Cavalot, A. Bosia, M. Trovati] It has been hypothesized that some genetic factors link different conditions characterized by the presence of insulin resistance: among them, obesity, type 2 (non-insulin-dependent) diabetes mellitus and arterial hypertension. A good candidate could be the Na+/H+ exchanger, the increased activity of which is considered a genetic marker of essential hypertension. In this study we looked at whether the Na+ dependence of the Na+/H+ antiporter is modified in obese and type 2 diabetic patients, in the absence of arterial hypertension. The activity of this ion exchanger was measured in peripheral blood lymphocytes by acidifying them in Na+-free buffer and then monitoring the recovery of intracellular pH after Na+ addition. Quiescent lymphocytes were used because they do not have insulin receptors, thus ruling out the effects of the elevated insulin concentrations on the Na+/H+ exchanger activity. Antiport activity, measured as the ability to extrude H+ in the presence of external Na+, showed no differences in normotensive obese and type 2 diabetic patients when compared with healthy subjects. Our data therefore suggest that an altered Na+/H+ exchange activity cannot be considered a common feature of insulin-resistant states. Springer-Verlag, 1992 Arterial hypertension nationallicence Na+/H+ antiport nationallicence Obesity nationallicence Peripheral blood lymphocytes nationallicence Type 2 diabetes nationallicence Ghigo D. Department of Genetics, Biology and Medical Chemistry, University of Turin, Via Santena 5/bis, I-10126, Turin, Italy aut Alessio P. Department of Genetics, Biology and Medical Chemistry, University of Turin, Via Santena 5/bis, I-10126, Turin, Italy aut Burzacca S. Department of Clinical and Biological Sciences, Diabetic Unit, University of Turin, Italy aut Costamagna C. Department of Genetics, Biology and Medical Chemistry, University of Turin, Via Santena 5/bis, I-10126, Turin, Italy aut Anfossi G. Department of Clinical and Biological Sciences, Diabetic Unit, University of Turin, Italy aut Cavalot F. Department of Clinical and Biological Sciences, Diabetic Unit, University of Turin, Italy aut Bosia A. Department of Genetics, Biology and Medical Chemistry, University of Turin, Via Santena 5/bis, I-10126, Turin, Italy aut Trovati M. Department of Clinical and Biological Sciences, Diabetic Unit, University of Turin, Italy aut Acta Diabetologica Springer-Verlag 29/3-4(1992-09-01), 237-239 0940-5429 29:3-4<237 1992 29 592 https://doi.org/10.1007/BF00573496 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00573496 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ghigo D. Department of Genetics, Biology and Medical Chemistry, University of Turin, Via Santena 5/bis, I-10126, Turin, Italy aut NATIONALLICENCE 700 1- Alessio P. Department of Genetics, Biology and Medical Chemistry, University of Turin, Via Santena 5/bis, I-10126, Turin, Italy aut NATIONALLICENCE 700 1- Burzacca S. Department of Clinical and Biological Sciences, Diabetic Unit, University of Turin, Italy aut NATIONALLICENCE 700 1- Costamagna C. Department of Genetics, Biology and Medical Chemistry, University of Turin, Via Santena 5/bis, I-10126, Turin, Italy aut NATIONALLICENCE 700 1- Anfossi G. Department of Clinical and Biological Sciences, Diabetic Unit, University of Turin, Italy aut NATIONALLICENCE 700 1- Cavalot F. Department of Clinical and Biological Sciences, Diabetic Unit, University of Turin, Italy aut NATIONALLICENCE 700 1- Bosia A. Department of Genetics, Biology and Medical Chemistry, University of Turin, Via Santena 5/bis, I-10126, Turin, Italy aut NATIONALLICENCE 700 1- Trovati M. Department of Clinical and Biological Sciences, Diabetic Unit, University of Turin, Italy aut NATIONALLICENCE 773 0- Acta Diabetologica Springer-Verlag 29/3-4(1992-09-01), 237-239 0940-5429 29:3-4<237 1992 29 592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer