caa a22 4500 469128763 CHVBK 20180323133158.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00572552 doi (NATIONALLICENCE)springer-10.1007/BF00572552 Barium-cross-linked alginate beads: a simple, one-step method for successful immunoisolated transplantation of islets of Langerhans [Elektronische Daten] [T. Zekorn, A. Horcher, U. Siebers, R. Schnettler, G. Klöck, B. Hering, U. Zimmermann, R. Bretzel, K. Federlin] Transplantation of xenogeneic islets in immunoisolating membranes may solve the problems of the availability of human donor organs and long-term immunosuppression. Alginates are widely used for microencapsulation of isolated islets. This study presents data of a new method in which alginate was cross-linked with barium ions. In the perifusion experiment microencapsulated rat islets showed a biphasic insulin release with a short delay of the first phase. During static glucose challenge the insulin release ranged from 40% to 70% compared to free floating controls. In 3 of 11 diabetic mice, transplantation of 800 barium-alginate-bead encapsulated rat islets resulted in a non-fasting normoglycaemia at least up to 70 days. In the same model, transplantation of 3000 encapsulated porcine islets resulted in 6 of 10 recipients in normoglycaemia up to day 70, while 3 animals were still normoglycaemic on day 100. On day 21 after transplantation of encapsulated rat and porcine islets and intraperitoneal glucose tolerance test revealed rapid glucose assimilation in both groups. Histological examination demonstrated well-preserved islets at the end of the experiments. Immunohistological B-cell staining revealed the absence of recruitment of β-cells in the recipient's own pancreas. The barium-alginate microencapsulation method represents a simple one-step method for effective immunoisolated transplantation of large-scale islet preparations. Springer-Verlag, 1992 Bioartificial pancreas nationallicence Porcine islets nationallicence Rat islets nationallicence Xenogeneic transplantation nationallicence Alginate nationallicence Zekorn T. Medizinische Klinik III und Poliklinik, Justus Liebig-Universität, Rodthohl 6, W-6300, Giessen, Germany aut Horcher A. Medizinische Klinik III und Poliklinik, Justus Liebig-Universität, Rodthohl 6, W-6300, Giessen, Germany aut Siebers U. Medizinische Klinik III und Poliklinik, Justus Liebig-Universität, Rodthohl 6, W-6300, Giessen, Germany aut Schnettler R. Lehrstuhl für Biotechnologie der Universität, Am Hubland, W-8700, Würzburg, Germany aut Klöck G. Lehrstuhl für Biotechnologie der Universität, Am Hubland, W-8700, Würzburg, Germany aut Hering B. Medizinische Klinik III und Poliklinik, Justus Liebig-Universität, Rodthohl 6, W-6300, Giessen, Germany aut Zimmermann U. Lehrstuhl für Biotechnologie der Universität, Am Hubland, W-8700, Würzburg, Germany aut Bretzel R. Medizinische Klinik III und Poliklinik, Justus Liebig-Universität, Rodthohl 6, W-6300, Giessen, Germany aut Federlin K. Medizinische Klinik III und Poliklinik, Justus Liebig-Universität, Rodthohl 6, W-6300, Giessen, Germany aut Acta Diabetologica Springer-Verlag 29/2(1992-06-01), 99-106 0940-5429 29:2<99 1992 29 592 https://doi.org/10.1007/BF00572552 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00572552 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zekorn T. Medizinische Klinik III und Poliklinik, Justus Liebig-Universität, Rodthohl 6, W-6300, Giessen, Germany aut NATIONALLICENCE 700 1- Horcher A. Medizinische Klinik III und Poliklinik, Justus Liebig-Universität, Rodthohl 6, W-6300, Giessen, Germany aut NATIONALLICENCE 700 1- Siebers U. Medizinische Klinik III und Poliklinik, Justus Liebig-Universität, Rodthohl 6, W-6300, Giessen, Germany aut NATIONALLICENCE 700 1- Schnettler R. Lehrstuhl für Biotechnologie der Universität, Am Hubland, W-8700, Würzburg, Germany aut NATIONALLICENCE 700 1- Klöck G. Lehrstuhl für Biotechnologie der Universität, Am Hubland, W-8700, Würzburg, Germany aut NATIONALLICENCE 700 1- Hering B. Medizinische Klinik III und Poliklinik, Justus Liebig-Universität, Rodthohl 6, W-6300, Giessen, Germany aut NATIONALLICENCE 700 1- Zimmermann U. Lehrstuhl für Biotechnologie der Universität, Am Hubland, W-8700, Würzburg, Germany aut NATIONALLICENCE 700 1- Bretzel R. Medizinische Klinik III und Poliklinik, Justus Liebig-Universität, Rodthohl 6, W-6300, Giessen, Germany aut NATIONALLICENCE 700 1- Federlin K. Medizinische Klinik III und Poliklinik, Justus Liebig-Universität, Rodthohl 6, W-6300, Giessen, Germany aut NATIONALLICENCE 773 0- Acta Diabetologica Springer-Verlag 29/2(1992-06-01), 99-106 0940-5429 29:2<99 1992 29 592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer