caa a22 4500 46912895X CHVBK 20180323133158.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00779005 doi (NATIONALLICENCE)springer-10.1007/BF00779005 Composition of human islet cell preparations for transplantation [Elektronische Daten] [C. Sever, A. Demetris, J. Zeng, P. Carroll, A. Tzakis, J. Fung, T. Starzl, C. Ricordi] To study the cellular composition of human islet cell isolates for transplantation, formalin-fixed and paraffin-embedded cell pellets were stained by the immunoperoxidase method with a panel of antibodies characterising endocrine, epithelial, soft tissue and haematolymphoid components. Immediately after separation, the isolates contained 30-80% islet cells, differing mainly in the content of islet and acinar cells, whereas the soft tissue, ductal/ductular and haematolymphoid elements comprised a relatively constant 10-20%. After 1 week in culture the islet cell content of less highly purified isolates (30-40% islets) dropped dramatically to 5%. The highly purified isolates (70-80% islets) showed only a minimal change in cellular composition; however, approximately two-thirds of islet cells were degranulated and did not stain for insulin. Haematolymphoid components were still present in all cultured isolates. We conclude that primarily mechanical purification methods and short-term culture are not sufficient to eliminate highly immunogenic cells. In addition, short-term culture is deleterious to the isolate if a significant number of acinar cells is still present after enrichment. Springer-Verlag, 1992 Islets of Langerhand nationallicence Transplantation nationallicence Cell isolation nationallicence Immunoperoxidase techniques nationallicence Sever C. Department of Pathology, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut Demetris A. Department of Pathology, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut Zeng J. Division of Transplantation and Surgery, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut Carroll P. Division of Transplantation and Surgery, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut Tzakis A. Division of Transplantation and Surgery, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut Fung J. Division of Transplantation and Surgery, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut Starzl T. Division of Transplantation and Surgery, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut Ricordi C. Division of Transplantation and Surgery, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut Acta Diabetologica Springer-Verlag 28/3-4(1992-09-01), 233-238 0940-5429 28:3-4<233 1992 28 592 https://doi.org/10.1007/BF00779005 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00779005 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sever C. Department of Pathology, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Demetris A. Department of Pathology, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Zeng J. Division of Transplantation and Surgery, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Carroll P. Division of Transplantation and Surgery, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Tzakis A. Division of Transplantation and Surgery, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Fung J. Division of Transplantation and Surgery, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Starzl T. Division of Transplantation and Surgery, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Ricordi C. Division of Transplantation and Surgery, Pittsburgh Transplant Institute, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 773 0- Acta Diabetologica Springer-Verlag 28/3-4(1992-09-01), 233-238 0940-5429 28:3-4<233 1992 28 592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer