caa a22 4500 469128976 CHVBK 20180323133158.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00778997 doi (NATIONALLICENCE)springer-10.1007/BF00778997 Fat-induced changes in mouse pancreatic islet insulin secretion, insulin biosynthesis and glucose metabolism [Elektronische Daten] [K. Capito, S. Hansen, C. Hedeskov, H. Islin, P. Thams] Insulin secretion, insulin biosynthesis and islet glucose oxidation were studied in pancreatic islets isolated from fat-fed diabetic mice of both sexes. Insulin secretion from isolated islets was studied after consecutive stimulation with α-ketoisocaproic acid + glutamine, glucose, forskolin, and 12-O-tetradecanoylphorbol 13-acetate. Glucose-induced insulin secretion was impaired in islets from fat-fed mice. This was associated with a reduction of approximately 50% in islet glucose oxidation. Islet insulin secretion stimulated by the non-carbohydrate secretagogues tended to be higher in the fat-fed mice, but a statistically significant effect was not observed. Pancreatic insulin content was reduced by 50%, whereas the islet insulin and DNA content was unchanged after fat feeding. Proinsulin mRNA was reduced by 35% in islets from fat-fed mice, and was associated with a reduction of approximately 50% in glucose-stimulated (pro)insulin biosynthesis. It is concluded that the insulin secretory response of islets isolated from fat-fed mice is similar to the secretory pattern known from human type 2, non-insulin-dependent diabetics, and that a defect in islet glucose recognition, resulting in decreased glucose oxidation, may be responsible for the observed insulin secretory and biosynthetic defects seen after glucose stimulation. Springer-Verlag, 1992 Type 2, non-insulin-dependent diabetes mellitus nationallicence Insulin secretion nationallicence Islet glucose metabolism nationallicence Insulin biosynthesis nationallicence Proinsulin mRNA nationallicence Capito K. Department of Biochemistry A, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark aut Hansen S. Department of Biochemistry A, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark aut Hedeskov C. Department of Biochemistry A, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark aut Islin H. Department of Biochemistry A, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark aut Thams P. Department of Biochemistry A, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark aut Acta Diabetologica Springer-Verlag 28/3-4(1992-09-01), 193-198 0940-5429 28:3-4<193 1992 28 592 https://doi.org/10.1007/BF00778997 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00778997 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Capito K. Department of Biochemistry A, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark aut NATIONALLICENCE 700 1- Hansen S. Department of Biochemistry A, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark aut NATIONALLICENCE 700 1- Hedeskov C. Department of Biochemistry A, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark aut NATIONALLICENCE 700 1- Islin H. Department of Biochemistry A, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark aut NATIONALLICENCE 700 1- Thams P. Department of Biochemistry A, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark aut NATIONALLICENCE 773 0- Acta Diabetologica Springer-Verlag 28/3-4(1992-09-01), 193-198 0940-5429 28:3-4<193 1992 28 592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer