caa a22 4500 46912959X CHVBK 20180323133200.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00324648 doi (NATIONALLICENCE)springer-10.1007/BF00324648 Flame atomic absorption spectrometric determination of cadmium and copper in biological reference materials using on-line sorbent extraction preconcentration [Elektronische Daten] [Shukun Xu, Michael Sperling, Bernhard Welz] Summary: Cadmium and copper at the μg/g to ng/g level in plant and animal tissue reference materials, and at the μg/l level in urine were determined by flame atomic absorption spectrometry using on-line sorbent extraction preconcentration based on flow injection techniques. Bonded silica reversed phase sorbent with octadecyl functional groups (RP-C 18), packed in a 100 μl column, was used to collect the diethylammonium-N,N-diethyldithiocarbamate (DDTC) complex formed on-line in the sample digests at low pH. Methanol was used to elute the analyte chelates directly into the nebulizer-burner system of the spectrometer. Small air segments introduced before and after elution prevented the eluent from mixing with the sample solution and increased the sensitivity. A sampling frequency of 85/h could be obtained with a sample loading time of 30 s at a flow rate of 4.0 ml/min. The enrichment factor for both elements was 20 and the enhancement factors, including the effect of the organic solvent and with the flow spoiler removed, were 126 and 114 for cadmium and copper, respectively. The detection limits (3σ) were 0.15 μg/l for cadmium and 0.2 μg/l for copper. The precision was 2.3% and 1.4% r.s.d. for 10 μg/l Cd and 45 μg/l Cu, respectively (n=11). Results for the determination of cadmium and copper in various biological reference materials were typically in good agreement with certified values. Low recoveries were observed, however, for cadmium in samples containing high levels of copper and/or iron, such as bovine liver. Springer-Verlag, 1992 Xu Shukun Department of Applied Research, Bodenseewerk Perkin-Elmer GmbH, W-7770, Überlingen, Federal Republic of Germany aut Sperling Michael Department of Applied Research, Bodenseewerk Perkin-Elmer GmbH, W-7770, Überlingen, Federal Republic of Germany aut Welz Bernhard Department of Applied Research, Bodenseewerk Perkin-Elmer GmbH, W-7770, Überlingen, Federal Republic of Germany aut Fresenius' Journal of Analytical Chemistry Springer-Verlag 344/12(1992-12-01), 535-540 0937-0633 344:12<535 1992 344 216 https://doi.org/10.1007/BF00324648 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00324648 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Shukun Department of Applied Research, Bodenseewerk Perkin-Elmer GmbH, W-7770, Überlingen, Federal Republic of Germany aut NATIONALLICENCE 700 1- Sperling Michael Department of Applied Research, Bodenseewerk Perkin-Elmer GmbH, W-7770, Überlingen, Federal Republic of Germany aut NATIONALLICENCE 700 1- Welz Bernhard Department of Applied Research, Bodenseewerk Perkin-Elmer GmbH, W-7770, Überlingen, Federal Republic of Germany aut NATIONALLICENCE 773 0- Fresenius' Journal of Analytical Chemistry Springer-Verlag 344/12(1992-12-01), 535-540 0937-0633 344:12<535 1992 344 216 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer