caa a22 4500 475737814 CHVBK 20180406123451.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s004300050006 doi (NATIONALLICENCE)springer-10.1007/s004300050006 Monitoring of cell-free viral DNA in primary Epstein-Barr virus infection [Elektronische Daten] [Hiroshi Kimura, Kazuo Nishikawa, Yo Hoshino, Ayako Sofue, Yukihiro Nishiyama, Tsuneo Morishima] Primary Epstein-Barr virus (EBV) infection in childhood is usually asymptomatic, but sometimes causes infectious mononucleosis (IM). Very occasionally, IM develops into a life-threatening EBV-associated hemophagocytic syndrome (EBV-AHS). We studied the importance and usefulness of measuring cell-free viral DNA in the serum of patients with these primary EBV infections. Using real-time quantitative polymerase chain reaction, cell-free EBV-DNA was quantified in the serum of nine children with IM and three with EBV-AHS. In the acute phase of IM, an average of 102.4 copies/ml of EBV-DNA was detected in 95% of sera. The EBV load gradually decreased and disappeared within 1 month. Patients with EBV-AHS had an extremely high viral load in their sera (105.5-107.4 copies/ml). The viral load persisted longer in these patients, although it decreased in parallel with the improvement of symptoms. These results indicate that cell-free EBV-DNA was frequently detected in patients with primary EBV infection and could, therefore, be used as a marker for EBV infection. Measuring the cell-free EBV-DNA is useful for monitoring the primary EBV infection, especially in EBV-AHS. Springer-Verlag Berlin Heidelberg, 2000 Key words Epstein-Barr virus nationallicence Real-time quantitative polymerase chain reaction nationallicence Infectious mononucleosis nationallicence Epstein-Barr virus-associated hemophagocytic syndrome nationallicence Kimura Hiroshi Department of Pediatrics, Nagoya University School of Medicine, 65 Tsuruma-cho, 466-8550, Showa-ku, Nagoya, Japan aut Nishikawa Kazuo Department of Pediatrics, Ekisaikai General Hospital, Nagoya, Japan aut Hoshino Yo Department of Pediatrics, Nagoya University School of Medicine, 65 Tsuruma-cho, 466-8550, Showa-ku, Nagoya, Japan aut Sofue Ayako Department of Pediatrics, Okazaki City Hospital, Aichi, Japan aut Nishiyama Yukihiro Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya, Japan aut Morishima Tsuneo Department of Pediatrics, Nagoya University School of Medicine, 65 Tsuruma-cho, 466-8550, Showa-ku, Nagoya, Japan aut https://doi.org/10.1007/s004300050006 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004300050006 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kimura Hiroshi Department of Pediatrics, Nagoya University School of Medicine, 65 Tsuruma-cho, 466-8550, Showa-ku, Nagoya, Japan aut NATIONALLICENCE 700 1- Nishikawa Kazuo Department of Pediatrics, Ekisaikai General Hospital, Nagoya, Japan aut NATIONALLICENCE 700 1- Hoshino Yo Department of Pediatrics, Nagoya University School of Medicine, 65 Tsuruma-cho, 466-8550, Showa-ku, Nagoya, Japan aut NATIONALLICENCE 700 1- Sofue Ayako Department of Pediatrics, Okazaki City Hospital, Aichi, Japan aut NATIONALLICENCE 700 1- Nishiyama Yukihiro Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya, Japan aut NATIONALLICENCE 700 1- Morishima Tsuneo Department of Pediatrics, Nagoya University School of Medicine, 65 Tsuruma-cho, 466-8550, Showa-ku, Nagoya, Japan aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer