caa a22 4500 475737822 CHVBK 20180406123451.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s004300000037 doi (NATIONALLICENCE)springer-10.1007/s004300000037 Incomplete replication of human parainfluenza virus type 4 in LLC-MK2 cells and in L929 cells [Elektronische Daten] [Hiroshi Komada, Hisae Inoue, Chihiro Yamabayashi, Morihiro Ito, Mitsuo Kawano, Machiko Nishio, Masato Tsurudome, Yuji Kozuka, Naoya Noda, Kazuyoshi Namba, Myles O'Brien, Yasuhiko Ito] Human parainfluenza virus type 4A (hPIV-4A) and type 4B (hPIV-4B) were tested for their ability to replicate in the monkey kidney LLC-MK2 cell line (MK2 cells) and the murine L929 cell line (L929 cells). These cells are normally non-permissive for replication of hPIV-4; however, treatment with acetylated trypsin led to virus replication in MK2 cells, but was less effective for L929 cells. Endogenously produced interferon (IFN) played no role in virus replication in L929 cells. Synthesis of virus-specific polypeptides was suppressed in L929 cells. WhereasNP-mRNA and HN-mRNA were detected in MK2 cells, no HN-mRNA was detected in L929 cells. These results indicate that hPIV-4 can infect both MK2 cells and L929 cells. In MK2 cells, when protease exists in the extracellular medium, hPIV-4 exhibits multistep growth. In L929 cells, however, the cause of incomplete replication might be lack of other unknown factors. Springer-Verlag Berlin Heidelberg, 2000 Key words Human parainfluenza virus type 4 nationallicence Incomplete replication nationallicence LLC-MK2 cell line nationallicence L929 cell line nationallicence Komada Hiroshi Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut Inoue Hisae Department of Microbiology, Suzuka University of Medical Science, 510-0293, Suzuka, Mie, Japan aut Yamabayashi Chihiro Department of Microbiology, Suzuka University of Medical Science, 510-0293, Suzuka, Mie, Japan aut Ito Morihiro Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut Kawano Mitsuo Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut Nishio Machiko Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut Tsurudome Masato Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut Kozuka Yuji Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut Noda Naoya Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut Namba Kazuyoshi Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut O'Brien Myles Mie Prefectural College of Nursing, 514-0116, Tsu, Mie, Japan aut Ito Yasuhiko Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut https://doi.org/10.1007/s004300000037 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004300000037 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Komada Hiroshi Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Inoue Hisae Department of Microbiology, Suzuka University of Medical Science, 510-0293, Suzuka, Mie, Japan aut NATIONALLICENCE 700 1- Yamabayashi Chihiro Department of Microbiology, Suzuka University of Medical Science, 510-0293, Suzuka, Mie, Japan aut NATIONALLICENCE 700 1- Ito Morihiro Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Kawano Mitsuo Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Nishio Machiko Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Tsurudome Masato Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Kozuka Yuji Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Noda Naoya Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Namba Kazuyoshi Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- O'Brien Myles Mie Prefectural College of Nursing, 514-0116, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Ito Yasuhiko Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer