caa a22 4500 475737830 CHVBK 20180406123451.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s004309900030 doi (NATIONALLICENCE)springer-10.1007/s004309900030 Site-specific invasion of the basal ganglia by Nocardia asteroides GUH-2 [Elektronische Daten] [Blaine L. Beaman, Donald Canfield, John Anderson, Brian Pate, Donald Calne] Nocardia asteroides GUH-2 (GUH-2) invades the nigrostriatal region of the brain in mice [15]. Selective dopaminergic neuronal dropout in the substantia nigra results in parkinsonian changes characterized by movement disorders responsive to L-dopa [15]. This is the only reported example of an experimental bacterial model for parkinsonism. Following i.v. inoculation of GUH-2 into the non-human primate Macaca fasicularius, the nocardiae preferentially invaded and grew within the basal ganglia (substantia nigra, caudate, putamen, and globus pallidus) often without inducing apparent inflammation. Reduced, limited growth of nocardiae occurred in the white matter of the cerebral cortex, medulla, and hippocampus, whereas neither significant adherence to nor growth within the meninges was observed. Twenty-four hours after injection, nocardial cells were found within capillary endothelial cells, the basal lamina, neurons, astroglia and in axonal extensions. The bacteria, in endothelial cells, were surrounded by a unit membrane, but in the basal lamina they appeared to be free and not membrane bound. After the organisms passed into the brain parenchyma, the nocardiae once again became surrounded by membrane, often being encapsulated by numerous layers with the innermost layer tightly adherence to the bacterial surface. There was a propensity for nocardial growth within and along myelinated axons, either with or without disruption to the surrounding myelin sheath. There was electron microscopic evidence that the nocardiae induced a neurodegenerative response especially in the substantia nigra region. Thus, the early interactions of GUH-2 within the primate brain appeared to be similar to those reported in the mouse. Springer-Verlag Berlin Heidelberg, 2000 Key words Nocardia nationallicence Basal ganglia nationallicence Substantia nigra nationallicence Neurodegeneration nationallicence Beaman Blaine L. Department of Medical Microbiology and Immunology, University of California School of Medicine, 95616, Davis, CA, USA aut Canfield Donald California Regional Primate Research Center (CRPRC), University of California, 95616, Davis, CA, USA aut Anderson John California Regional Primate Research Center (CRPRC), University of California, 95616, Davis, CA, USA aut Pate Brian Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada aut Calne Donald Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada aut https://doi.org/10.1007/s004309900030 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004309900030 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Beaman Blaine L. Department of Medical Microbiology and Immunology, University of California School of Medicine, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Canfield Donald California Regional Primate Research Center (CRPRC), University of California, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Anderson John California Regional Primate Research Center (CRPRC), University of California, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Pate Brian Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada aut NATIONALLICENCE 700 1- Calne Donald Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer