caa a22 4500 475737857 CHVBK 20180406123451.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s004300050007 doi (NATIONALLICENCE)springer-10.1007/s004300050007 There is no disease-specific role for streptococci-responsive synovial T lymphocytes in the pathogenesis of psoriatic arthritis [Elektronische Daten] [Henrike Thomssen, Boris Hoffmann, Marion Schank, Dirk Elewaut, Karl-Hermann Meyer zum Büschenfelde, Elisabeth Märker-Hermann] The initiation or exacerbation of psoriasis vulgaris is associated with infections by group A streptococci. T lymphocytes specific for streptococcal antigens or expressing a restricted, for streptococcal superantigens typical T cell receptor Vβ chain repertoire have been described in psoriatic skin lesions. The aim of our study was, therefore, to clarify whether streptococci-reactive T lymphocytes played a role in the pathogenesis of psoriatic arthritis (PsA), and by which antigens they might be stimulated. Synovial membrane mononuclear cells from patients with PsA and other arthropathies, separated by collagenase digestion, were expanded in interleukin-2-supplemented medium and subsequently cloned in a representative cloning procedure. The T cell lines and about 30% of the T cell clones proliferated in response to preparations of group A streptococci but not to other bacteria as tested by [3H]thymidine incorporation assays. Interestingly, they did not proliferate in response to exotoxin-negative streptococci, but did so in response to the streptococcal pyrogenic exotoxins A and C, which are known to be superantigens. Accordingly, no HLA-DR restriction was seen for the proliferative response. The remaining 70% of the established T cell clones did not react to an antigen of group A streptococci. Our results show that in patients with PsA, osteoarthritis or rheumatoid arthritis a significant number of synovial T lymphocytes were responsive to streptococcal superantigens, but not to conventional streptococcal antigens. A disease-specific role of streptococci-reactive T lymphocytes in the pathogenesis of PsA is, therefore, unlikely. Springer-Verlag Berlin Heidelberg, 2000 Key words Psoriatic arthritis nationallicence Synovial membrane nationallicence T lymphocytes nationallicence Streptococcal superantigen nationallicence Thomssen Henrike 1st Medical Department, University Hospital, Langenbeckstr. 1, 55101, Mainz, Deutschland aut Hoffmann Boris 1st Medical Department, University Hospital, Langenbeckstr. 1, 55101, Mainz, Deutschland aut Schank Marion 1st Medical Department, University Hospital, Langenbeckstr. 1, 55101, Mainz, Deutschland aut Elewaut Dirk Department of Rheumatology, University Hospital, de Pintelaan 185, 9000, Gent, Belgium aut Meyer zum Büschenfelde Karl-Hermann 1st Medical Department, University Hospital, Langenbeckstr. 1, 55101, Mainz, Deutschland aut Märker-Hermann Elisabeth 1st Medical Department, University Hospital, Langenbeckstr. 1, 55101, Mainz, Deutschland aut https://doi.org/10.1007/s004300050007 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004300050007 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thomssen Henrike 1st Medical Department, University Hospital, Langenbeckstr. 1, 55101, Mainz, Deutschland aut NATIONALLICENCE 700 1- Hoffmann Boris 1st Medical Department, University Hospital, Langenbeckstr. 1, 55101, Mainz, Deutschland aut NATIONALLICENCE 700 1- Schank Marion 1st Medical Department, University Hospital, Langenbeckstr. 1, 55101, Mainz, Deutschland aut NATIONALLICENCE 700 1- Elewaut Dirk Department of Rheumatology, University Hospital, de Pintelaan 185, 9000, Gent, Belgium aut NATIONALLICENCE 700 1- Meyer zum Büschenfelde Karl-Hermann 1st Medical Department, University Hospital, Langenbeckstr. 1, 55101, Mainz, Deutschland aut NATIONALLICENCE 700 1- Märker-Hermann Elisabeth 1st Medical Department, University Hospital, Langenbeckstr. 1, 55101, Mainz, Deutschland aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer