caa a22 4500 475737865 CHVBK 20180406123451.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/PL00008253 doi (NATIONALLICENCE)springer-10.1007/PL00008253 Frequent contamination of Chlamydia trachomatis and Chlamydia pneumoniae strains with mycoplasma. Biological relevance and selective eradication of mycoplasma from chlamydial cultures with mupirocin [Elektronische Daten] [Birgit Krauße-Opatz, Petra Dollmann, Henning Zeidler, Jens G. Kuipers, Lars Köhler] Several strains of Chlamydia trachomatis (CT) and C. pneumoniae (CP) from different sources were screened for mycoplasma contamination using a sensitive nested 16S rDNA polymerase chain reaction-specific for a broad range of mycoplasma species. Five of nine CT and 5/16 CP isolates were contaminated by mycoplasma. Mycoplasma fermentans, M. hyorhinis and M. hominis were found as contaminating agents. To our knowledge no data are available on whether coinfection of chlamydia with mycoplasma alters the biological behavior of chlamydia. Analysis of the biological effect of mycoplasma on chlamydial infection showed a profound mycoplasma-induced reduction of chlamydial growth. Mycoplasma were efficiently eliminated from chlamydial cultures in HEp-2 cells by treatment with mupirocin without affecting chlamydial replication or host cell growth. Two chlamydial strains, C. trachomatis serovar K and one clinical isolate of C. pneumoniae were purged by this method. Springer-Verlag Berlin Heidelberg, 2000 Key words Chlamydia nationallicence Mycoplasma nationallicence Coinfection nationallicence Krauße-Opatz Birgit Division of Rheumatology, Department of Internal Medicine, Medical School Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany e-mail: koehler.lars@mh-hannover.de Tel.: +49-511-5322190; Fax: +49-511-5325841, DE aut Dollmann Petra Division of Rheumatology, Department of Internal Medicine, Medical School Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany e-mail: koehler.lars@mh-hannover.de Tel.: +49-511-5322190; Fax: +49-511-5325841, DE aut Zeidler Henning Division of Rheumatology, Department of Internal Medicine, Medical School Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany e-mail: koehler.lars@mh-hannover.de Tel.: +49-511-5322190; Fax: +49-511-5325841, DE aut Kuipers Jens G. Division of Rheumatology, Department of Internal Medicine, Medical School Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany e-mail: koehler.lars@mh-hannover.de Tel.: +49-511-5322190; Fax: +49-511-5325841, DE aut Köhler Lars Division of Rheumatology, Department of Internal Medicine, Medical School Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany e-mail: koehler.lars@mh-hannover.de Tel.: +49-511-5322190; Fax: +49-511-5325841, DE aut https://doi.org/10.1007/PL00008253 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00008253 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Krauße-Opatz Birgit Division of Rheumatology, Department of Internal Medicine, Medical School Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany e-mail: koehler.lars@mh-hannover.de Tel.: +49-511-5322190; Fax: +49-511-5325841, DE aut NATIONALLICENCE 700 1- Dollmann Petra Division of Rheumatology, Department of Internal Medicine, Medical School Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany e-mail: koehler.lars@mh-hannover.de Tel.: +49-511-5322190; Fax: +49-511-5325841, DE aut NATIONALLICENCE 700 1- Zeidler Henning Division of Rheumatology, Department of Internal Medicine, Medical School Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany e-mail: koehler.lars@mh-hannover.de Tel.: +49-511-5322190; Fax: +49-511-5325841, DE aut NATIONALLICENCE 700 1- Kuipers Jens G. Division of Rheumatology, Department of Internal Medicine, Medical School Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany e-mail: koehler.lars@mh-hannover.de Tel.: +49-511-5322190; Fax: +49-511-5325841, DE aut NATIONALLICENCE 700 1- Köhler Lars Division of Rheumatology, Department of Internal Medicine, Medical School Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany e-mail: koehler.lars@mh-hannover.de Tel.: +49-511-5322190; Fax: +49-511-5325841, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer