caa a22 4500 475737873 CHVBK 20180406123451.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/PL00008251 doi (NATIONALLICENCE)springer-10.1007/PL00008251 N -Glycosylation contributes to the limited cross-reactivity between hemagglutinin neuraminidase proteins of human parainfluenza virus type 4A and 4B [Elektronische Daten] [Hiroshi Komada, Morihiro Ito, Machiko Nishio, Mitsuo Kawano, Hisataka Ohta, Masato Tsurudome, Shigeru Kusagawa, Myles O'Brien, Hisanori Bando, Yasuhiko Ito] cDNAs encoding human parainfluenza virus type 4B (hPIV-4B) hemagglutinin neuraminidase (HN) protein were cloned and the nucleotide sequences were determined. A high degree of identity (81.4%) was observed between the nucleotide sequences of hPIV-4A and -4B HN proteins, and an 87.3% identity was found between the deduced amino acid sequences. This degree of identity is considered to be greater than immunological similarity between hPIV-4A and -4B HN proteins determined using monoclonal antibodies. To elucidate the causes of the antigenic difference between HN proteins of hPIV-4A and -4B, we constructed three cDNAs of hPIV-4B HN whose potential N-glycosylation sites were partially or completely the same as in hPIV-4A HN cDNA. We compared the antigenicity of the expressed wild-type and mutant proteins, and found that the antigenicities of the mutant hPIV-4B HN proteins were more similar to the hPIV-4A HN protein than to the non-mutant hPIV-4B HN protein. This study indicated that the antigenic diversity between hPIV-4A and -4B was partly caused by deletion or creation of glycosylation sites, showing that the point mutations resulting in deletion or creation of glycosylation sites is one of the initial steps leading to the division of virus into subtypes. Springer-Verlag Berlin Heidelberg, 2000 Key words Human parainfluenza virus type 4 hemagglutinin neuraminidase protein nationallicence N-Glycosylation site nationallicence Antigenicity nationallicence Komada Hiroshi Department of Microbiology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan e-mail: ito@doc.medic.mie-u.ac.jp; Fax: +81-59-2315008, JP aut Ito Morihiro Department of Microbiology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan e-mail: ito@doc.medic.mie-u.ac.jp; Fax: +81-59-2315008, JP aut Nishio Machiko Department of Microbiology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan e-mail: ito@doc.medic.mie-u.ac.jp; Fax: +81-59-2315008, JP aut Kawano Mitsuo Department of Microbiology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan e-mail: ito@doc.medic.mie-u.ac.jp; Fax: +81-59-2315008, JP aut Ohta Hisataka Department of Biology, Faculty of Science, Science University of Tokyo, Noda, Chiba, 278-8510, Japan, JP aut Tsurudome Masato Department of Microbiology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan e-mail: ito@doc.medic.mie-u.ac.jp; Fax: +81-59-2315008, JP aut Kusagawa Shigeru Department of Microbiology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan e-mail: ito@doc.medic.mie-u.ac.jp; Fax: +81-59-2315008, JP aut O'Brien Myles Mie Prefectural College of Nursing, Tsu, Mie 514-0116, Japan, JP aut Bando Hisanori Department of Applied Bioscience, Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan, JP aut Ito Yasuhiko Department of Microbiology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan e-mail: ito@doc.medic.mie-u.ac.jp; Fax: +81-59-2315008, JP aut https://doi.org/10.1007/PL00008251 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00008251 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Komada Hiroshi Department of Microbiology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan e-mail: ito@doc.medic.mie-u.ac.jp; Fax: +81-59-2315008, JP aut NATIONALLICENCE 700 1- Ito Morihiro Department of Microbiology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan e-mail: ito@doc.medic.mie-u.ac.jp; Fax: +81-59-2315008, JP aut NATIONALLICENCE 700 1- Nishio Machiko Department of Microbiology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan e-mail: ito@doc.medic.mie-u.ac.jp; Fax: +81-59-2315008, JP aut NATIONALLICENCE 700 1- Kawano Mitsuo Department of Microbiology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan e-mail: ito@doc.medic.mie-u.ac.jp; Fax: +81-59-2315008, JP aut NATIONALLICENCE 700 1- Ohta Hisataka Department of Biology, Faculty of Science, Science University of Tokyo, Noda, Chiba, 278-8510, Japan, JP aut NATIONALLICENCE 700 1- Tsurudome Masato Department of Microbiology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan e-mail: ito@doc.medic.mie-u.ac.jp; Fax: +81-59-2315008, JP aut NATIONALLICENCE 700 1- Kusagawa Shigeru Department of Microbiology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan e-mail: ito@doc.medic.mie-u.ac.jp; Fax: +81-59-2315008, JP aut NATIONALLICENCE 700 1- O'Brien Myles Mie Prefectural College of Nursing, Tsu, Mie 514-0116, Japan, JP aut NATIONALLICENCE 700 1- Bando Hisanori Department of Applied Bioscience, Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan, JP aut NATIONALLICENCE 700 1- Ito Yasuhiko Department of Microbiology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan e-mail: ito@doc.medic.mie-u.ac.jp; Fax: +81-59-2315008, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer