caa a22 4500 47573789X CHVBK 20180406123451.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/PL00008255 doi (NATIONALLICENCE)springer-10.1007/PL00008255 GyrA sequence-based typing of Legionella [Elektronische Daten] [Alix Feddersen, Heinz-Georg W. Meyer, Philipp Matthes, Sucharit Bhakdi, Matthias Husmann] Comparative sequence analysis of a 423-bp segment of the gyrA gene including a region homologous to the quinolone resistance-determining region (QRDR) of other species was evaluated as a novel typing method for Legionella strains. The study was performed with 29 reference strains representing 11 different Legionella species, with various serogroups, and with 13 clinical isolates of L. pneumophila. Pulsed-field gel electrophoresis and serotyping were employed for comparison of the clinical isolates. QRDR sequencing proved to be a highly discriminative tool for typing Legionellae, and permitted identification of species, serogroups and even different strains within serogroup 1. None of the isolates were resistant to quinolones in vitro and this correlated with dissence of mutations in the QRDR region. The data show that comparative sequence analysis of a short fragment of the gyrA gene is a potentially useful tool for typing of Legionella beyond the serogroup level. It is anticipated that mutations of the QRDR may arise in Legionella as a consequence of the introduction of quinolones as the agents of choice for the treatment of infections with this agent in immunocompromised patients. The employment of QRDR-typing maybe helpful in uncovering such mutations. Springer-Verlag Berlin Heidelberg, 2000 Key wordsLegionella nationallicence GyrA nationallicence Typing nationallicence Quinolone resistance-determining region nationallicence Pulsed-field gel electrophoresis nationallicence Feddersen Alix Institute of Medical Microbiology und Hygiene, Johannes Gutenberg-University of Mainz, Hochhaus am Augustusplatz, 55101 Mainz, Germany e-mail: makowiec@mail.uni-mainz.de Tel.: +49-6131-177342; Fax: +49-6131-3932359, DE aut Meyer Heinz-Georg W. Institute of Medical Microbiology und Hygiene, Johannes Gutenberg-University of Mainz, Hochhaus am Augustusplatz, 55101 Mainz, Germany e-mail: makowiec@mail.uni-mainz.de Tel.: +49-6131-177342; Fax: +49-6131-3932359, DE aut Matthes Philipp Institute of Medical Microbiology und Hygiene, Johannes Gutenberg-University of Mainz, Hochhaus am Augustusplatz, 55101 Mainz, Germany e-mail: makowiec@mail.uni-mainz.de Tel.: +49-6131-177342; Fax: +49-6131-3932359, DE aut Bhakdi Sucharit Institute of Medical Microbiology und Hygiene, Johannes Gutenberg-University of Mainz, Hochhaus am Augustusplatz, 55101 Mainz, Germany e-mail: makowiec@mail.uni-mainz.de Tel.: +49-6131-177342; Fax: +49-6131-3932359, DE aut Husmann Matthias Institute of Medical Microbiology und Hygiene, Johannes Gutenberg-University of Mainz, Hochhaus am Augustusplatz, 55101 Mainz, Germany e-mail: makowiec@mail.uni-mainz.de Tel.: +49-6131-177342; Fax: +49-6131-3932359, DE aut https://doi.org/10.1007/PL00008255 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00008255 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feddersen Alix Institute of Medical Microbiology und Hygiene, Johannes Gutenberg-University of Mainz, Hochhaus am Augustusplatz, 55101 Mainz, Germany e-mail: makowiec@mail.uni-mainz.de Tel.: +49-6131-177342; Fax: +49-6131-3932359, DE aut NATIONALLICENCE 700 1- Meyer Heinz-Georg W. Institute of Medical Microbiology und Hygiene, Johannes Gutenberg-University of Mainz, Hochhaus am Augustusplatz, 55101 Mainz, Germany e-mail: makowiec@mail.uni-mainz.de Tel.: +49-6131-177342; Fax: +49-6131-3932359, DE aut NATIONALLICENCE 700 1- Matthes Philipp Institute of Medical Microbiology und Hygiene, Johannes Gutenberg-University of Mainz, Hochhaus am Augustusplatz, 55101 Mainz, Germany e-mail: makowiec@mail.uni-mainz.de Tel.: +49-6131-177342; Fax: +49-6131-3932359, DE aut NATIONALLICENCE 700 1- Bhakdi Sucharit Institute of Medical Microbiology und Hygiene, Johannes Gutenberg-University of Mainz, Hochhaus am Augustusplatz, 55101 Mainz, Germany e-mail: makowiec@mail.uni-mainz.de Tel.: +49-6131-177342; Fax: +49-6131-3932359, DE aut NATIONALLICENCE 700 1- Husmann Matthias Institute of Medical Microbiology und Hygiene, Johannes Gutenberg-University of Mainz, Hochhaus am Augustusplatz, 55101 Mainz, Germany e-mail: makowiec@mail.uni-mainz.de Tel.: +49-6131-177342; Fax: +49-6131-3932359, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer