caa a22 4500 475737903 CHVBK 20180406123451.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/PL00008252 doi (NATIONALLICENCE)springer-10.1007/PL00008252 Enhanced transcription of the s-adenosylhomocysteine hydrolase gene precedes Epstein-Barr virus lytic gene activation in ganglioside-stimulated lymphoma cells [Elektronische Daten] [Lars Schaade, Michael Kleines, Bernd Krone, Michael Hausding, Robert Walter, Klaus Ritter] Stimulation of Epstein-Barr virus (EBV) genome-positive Burkitt lymphoma cells with the ganglioside IV3NeuAc-nLcOse4Cer leads to the induction of cell differentiation processes and activates the EBV lytic viral cycle. In cells of the Burkitt lymphoma line Raji differential expression of host cell genes was analysed in the early phase (150 min) post stimulation with the ganglioside to display the cell activities that precede the activation of the EBV lytic cycle using the differential display reverse transcription-polymerase chain reaction technique. Multiple fragment cDNAs derived from control cells and ganglioside-stimulated cells were amplified using random primers and displayed via polyacrylamide gel electrophoresis. The expression pattern of 8,400 bands was analysed. Eleven differentially expressed fragment cDNAs were reamplified and identified by nucleotide sequencing. Six of these could be identified as coding for proteins that may take part in virus reactivation and differentiation. The most striking finding was the induction of s-adenosylhomocysteine hydrolase (AHCY) expression. The cellular enzyme AHCY plays an important role in transmethylation reactions controlling the replication of several viruses. Thus, an involvement in EBV replication can be suggested. Springer-Verlag Berlin Heidelberg, 2000 Key words Differential display nationallicence Gene expression nationallicence Virus reactivation nationallicence Epstein-Barr virus nationallicence Schaade Lars Division of Virology, Department of Medical Microbiology, University Hospital RWTH Aachen, Pauwelsstrasse 30, 52074 Aachen, Germany e-mail: kritter@post.klinikum.rwth-aachen.de Tel.: +49-241-8088460; Fax: +49-241-8888483, DE aut Kleines Michael Division of Virology, Department of Medical Microbiology, University Hospital RWTH Aachen, Pauwelsstrasse 30, 52074 Aachen, Germany e-mail: kritter@post.klinikum.rwth-aachen.de Tel.: +49-241-8088460; Fax: +49-241-8888483, DE aut Krone Bernd Department of Virology, University of Göttingen, Kreuzbergring 57, 37075 Göttingen, Germany, DE aut Hausding Michael Department of Virology, University of Göttingen, Kreuzbergring 57, 37075 Göttingen, Germany, DE aut Walter Robert Department of Virology, University of Göttingen, Kreuzbergring 57, 37075 Göttingen, Germany, DE aut Ritter Klaus Division of Virology, Department of Medical Microbiology, University Hospital RWTH Aachen, Pauwelsstrasse 30, 52074 Aachen, Germany e-mail: kritter@post.klinikum.rwth-aachen.de Tel.: +49-241-8088460; Fax: +49-241-8888483, DE aut https://doi.org/10.1007/PL00008252 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00008252 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schaade Lars Division of Virology, Department of Medical Microbiology, University Hospital RWTH Aachen, Pauwelsstrasse 30, 52074 Aachen, Germany e-mail: kritter@post.klinikum.rwth-aachen.de Tel.: +49-241-8088460; Fax: +49-241-8888483, DE aut NATIONALLICENCE 700 1- Kleines Michael Division of Virology, Department of Medical Microbiology, University Hospital RWTH Aachen, Pauwelsstrasse 30, 52074 Aachen, Germany e-mail: kritter@post.klinikum.rwth-aachen.de Tel.: +49-241-8088460; Fax: +49-241-8888483, DE aut NATIONALLICENCE 700 1- Krone Bernd Department of Virology, University of Göttingen, Kreuzbergring 57, 37075 Göttingen, Germany, DE aut NATIONALLICENCE 700 1- Hausding Michael Department of Virology, University of Göttingen, Kreuzbergring 57, 37075 Göttingen, Germany, DE aut NATIONALLICENCE 700 1- Walter Robert Department of Virology, University of Göttingen, Kreuzbergring 57, 37075 Göttingen, Germany, DE aut NATIONALLICENCE 700 1- Ritter Klaus Division of Virology, Department of Medical Microbiology, University Hospital RWTH Aachen, Pauwelsstrasse 30, 52074 Aachen, Germany e-mail: kritter@post.klinikum.rwth-aachen.de Tel.: +49-241-8088460; Fax: +49-241-8888483, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer