caa a22 4500 475737911 CHVBK 20180406123451.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004300000047 doi (NATIONALLICENCE)springer-10.1007/s004300000047 DNA vaccination using coexpression of cytokine genes with a bacterial gene encoding a 60-kDa heat shock protein [Elektronische Daten] [Mathias W. Hornef, Annette Noll, Reinhold Schirmbeck, Jörg Reimann, Ingo B. Autenrieth] Coexpression of cytokine genes together with antigen-encoding genes in DNA vaccination vectors can increase humoral and cellular immune responses and may steer them in a Th1 or Th2 direction. In this study, the modulatory effect of interleukin (IL)-2, IL-4, and interferon (IFN)-γ coexpressed with the 60-kDa heat shock protein (Hsp60) of Yersinia enterocolitica O:8 (Y-Hsp60) was studied. DNA vaccination with y-hsp60 evoked specific humoral and cellular immune responses as well as reduction of the splenic bacterial load upon challenge with Y. enterocolitica in a mouse infection model. Coexpression of IL-2 or IFN-γ enhanced Y. enterocolitica-specific total IgG (P < 0.05) and IgG2a antibody responses. Coexpression of IFN-γ also improved the proliferative T cell responses upon stimulation with Y-Hsp60. A reduction of the splenic bacterial load as compared with the plasmid encoding Y-Hsp60 only was found for the IFN-γ coexpressing vector. Thus, coexpression of cytokine genes such as IFN-γ in DNA vaccination vectors might improve immunity and help to overcome the side effects of standard adjuvants. Springer-Verlag Berlin Heidelberg, 2000 Key words DNA vaccination nationallicence Bacterial heat shock protein nationallicence Interleukin-2 nationallicence Interleukin-4 nationallicence Interferon-γ nationallicence Hornef Mathias W. Max von Pettenkofer Institute for Hygiene and Medical Microbiology, LMU Munich, Munich, Germany, DE aut Noll Annette Max von Pettenkofer Institute for Hygiene and Medical Microbiology, LMU Munich, Munich, Germany, DE aut Schirmbeck Reinhold Department of Microbiology and Hygiene, University of Ulm, Ulm, Germany, DE aut Reimann Jörg Department of Microbiology and Hygiene, University of Ulm, Ulm, Germany, DE aut Autenrieth Ingo B. Max von Pettenkofer Institute for Hygiene and Medical Microbiology, LMU Munich, Munich, Germany, DE aut https://doi.org/10.1007/s004300000047 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004300000047 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hornef Mathias W. Max von Pettenkofer Institute for Hygiene and Medical Microbiology, LMU Munich, Munich, Germany, DE aut NATIONALLICENCE 700 1- Noll Annette Max von Pettenkofer Institute for Hygiene and Medical Microbiology, LMU Munich, Munich, Germany, DE aut NATIONALLICENCE 700 1- Schirmbeck Reinhold Department of Microbiology and Hygiene, University of Ulm, Ulm, Germany, DE aut NATIONALLICENCE 700 1- Reimann Jörg Department of Microbiology and Hygiene, University of Ulm, Ulm, Germany, DE aut NATIONALLICENCE 700 1- Autenrieth Ingo B. Max von Pettenkofer Institute for Hygiene and Medical Microbiology, LMU Munich, Munich, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer